Lung Lysate (7 Days Old)

Supplier: PROSCI

1402-7
PRSI1402-7EA 153 EUR
PRSI1402-7
Lung Lysate (7 Days Old)
Cells

Lung tissue lysate (7 Days Old) was prepared by homogenisation in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7,4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0,1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin.


Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6,8, 12,5% glycerol, 1% sodium dodecylsulfate, 0,01% bromophenol blue) containing 5% β-mercaptoethanol.

New Product

Order Now

SPECIFICATIONS

Concentration 2 mg/ml
Environmentally Preferable
Tested applications WB
Storage buffer Lung lysate (7 Days Old) is supplied in SDS sample buffer containing 5% β-mercaptoethanol
Storage conditions Store at 2...8 °C for continuous use. For extended storage, freeze working aliquots at −70 °C.

Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Learn more About VWR