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You Searched For: Enzyme Assays

Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.

Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.


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Protease and peptidase substrate

Supplier: Biotium
Description: Fluorogenic substrates for proteases and peptidases.

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Superoxide dismutase assay kit

Supplier: Cayman Chemical
Description: Superoxide Dismutase Assay (SOD) kit is a fast and reliable assay for the measurement of SOD activity from plasma, serum, tissue homogenates and cell lysates.

Product Image-PIER88316

Serine hydrolase probes, ActivX™ FP

Supplier: Thermo Scientific
Description: The ActivX® FP serine hydrolase probes enable selective labeling and enrichment of active serine hydrolases. Fluorophosphonate (FP) probes specifically label serines of enzymatically active serine hydrolases. FP probes are also effective for screening small molecule inhibitors against enzymes derived from cell lysates, subcellular fractions, tissues and recombinant proteins. Depending on the active-site probe tag group and the enzyme class to be profiled, active site-labeled enzymes are detected and quantified by Western blot, fluorescent gel imaging or mass spectrometry.

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Pierce™ Gaussia Luciferase Flash Assay Kit

Supplier: Thermo Scientific
Description: The Thermo Scientific Pierce Gaussia Luciferase Flash Assay Kit provides researchers with the most sensitive assay for transcriptional activity of regulatory elements in mammalian cell culture media and whole cell lysate.

Product Image-786-431

SAM Methyltransferase assay, SAMfluoro™

Catalog Number: (786-431)
Supplier: G-Biosciences
Description: The SAMfluoro™ assay is a fluorescent, continuous enzyme coupled assay that can continuously monitor SAM-dependent methyltransferases without the use of radioactive labels or endpoint measurements.
UOM: 1 * 100 Assays
Product Image-786-440

Peroxide assay kit, PEROXsay™

Supplier: G-Biosciences
Description: PEROXsay™ is a colorimetric quantitative peroxide assay that measures the oxidation of ferrous (Fe²⁺+) ions to ferric (Fe³⁺) ions. Peroxides react with a sugar alcohol converting it to a peroxyl radical that subsequently starts the oxidation of ferrous ions to ferric ions. The acidic pH of the assay allows the ferric (Fe³⁺) ion to complex with the colorimetric reagent, resulting in a change in absorbance that is proportional to the peroxide concentration.

Product Image-OZBIGO10001

ONPG assay kit

Catalog Number: (OZBIGO10001)
Supplier: OZ BIOSCIENCES
Description: ONPG assay kit is ideal to quantify high expression level of beta-Gal.
UOM: 1 * 1 KIT
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Cypridina luciferase flash assay kit, Pierce™

Supplier: Thermo Scientific
Description: The Thermo Scientific Pierce Cypridina Luciferase Flash Assay Kit provides researchers with a highly sensitive assay for transcriptional activity of regulatory elements in mammalian cell culture media and whole cell lysate.

MSDS

Image Unavailable-PIER16165

Renilla luciferase flash assay kit, Pierce™, Thermo Scientific

Supplier: Thermo Scientific
Description: The Thermo Scientific Pierce Renilla Luciferase Flash Assay Kit provides researchers with a highly sensitive intracellular assay for transcriptional activity of regulatory elements in mammalian whole cell lysate.

Product Image-PIER23285

Quantitative peroxide assay kit (lipid), Pierce™

Catalog Number: (PIER23285)
Supplier: Thermo Scientific
Description: Pierce™ Quantitative Peroxide Assay Kit (Lipid) detects and measures hydrogen peroxide levels in biological samples using an iron and xylenol orange* (XO) reagent.
UOM: 1 * 1 KIT
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Renilla-firefly luciferase dual assay kit, Pierce™, Thermo Scientific

Supplier: Thermo Scientific
Description: The Thermo Scientific Pierce Renilla-Firefly Luciferase dual assay kit provides the necessary reagents to simultaneously detect intracellular Renilla and Red Firefly luciferase activity in mammalian whole cell lysates.

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Renilla luciferase glow assay kits, Pierce™

Supplier: Thermo Scientific
Description: The Thermo Scientific Pierce renilla Luciferase glow assay kit provides an extremely bright and stable bioluminescence signal (half-life approx. 3 hours), especially in the presence of green renilla luciferase reporter.

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Amplite® Colorimetric Acetylcholinesterase Assay Kit

Catalog Number: (AATB11400)
Supplier: AAT BIOQUEST
Description: Acetylcholinesterase, also known as AChE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group.
UOM: 1 * 200 Tests
Product Image-PIER75705

β-Galactosidase Assay Kit and Reagents

Supplier: Thermo Scientific
Description: The Mammalian β-Galactosidase Assay Reagent Kit is a simple and versatile assay to measure β-galactosidase activity in cultured mammalian cells. Cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single all-in-one lysis-and-assay solution. Alternatively, M-PER™ Mammalian Protein Extraction Reagent may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate used to assay for β-galactosidase activity. With M-PER Reagent, there is no need to scrape cells or to perform freeze-thaw cycles. The remaining lysates prepared with M-PER™ Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and tyrosine kinase), immunoassays (Western blot, ELISA, RIA) and protein purification.

Product Image-PIER75768

Yeast β-galactosidase assay kit, Pierce™

Catalog Number: (PIER75768)
Supplier: Thermo Scientific
Description: Yeast β-Galactosidase Assay Kit is specially formulated for yeast cells and ideal for identifying protein interactions<i> in vivo</i> using two-hybrid systems.
UOM: 1 * 1 KIT
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Gaussia-firefly luciferase dual assay kit, Pierce™, Thermo Scientific

Supplier: Thermo Scientific
Description: The Thermo Scientific Pierce Gaussia-Firefly Luciferase dual assay kit provides the necessary reagents to simultaneously detect intracellular Gaussia and red firefly luciferase activity in mammalian whole cell lysates.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
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