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You Searched For: Extraction Buffers

Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.

Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.


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Product Image-PIER78999

Y-PER™ plus Yeast Protein Extraction Reagent, Dialysable

Catalog Number: (PIER78999)
Supplier: Thermo Scientific
Description: This extraction reagent is formulated to effectively lyse yeast and bacterial cells to extract and solubilise proteins for analysis.
UOM: 1 * 500 mL
Product Image-A4150.0500

Lysis buffer, CTAB

Supplier: PanReac AppliChem
Description: Cationic detergent cetyltrimethylammonium bromide (CTAB) is used to liberate and complex with total cellular nucleic acids.

Product Image-PIER89901

RIPA lysis and extraction buffer, Pierce™

Supplier: Thermo Scientific
Description: The RIPA lysis and extraction buffer is a high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent for cultured mammalian cells.

Image Unavailable-OMEGRBB-1000

RBB Buffers

Supplier: OMEGA BIO-TEK
Description: RBB Buffer, 4000 ml, Omega Bio-Tek

Product Image-PIER89802

I-PER™ Insect Cell Protein Extraction Reagents

Catalog Number: (PIER89802)
Supplier: Thermo Scientific
Description: The I-PER Insect Cell Protein Extraction Reagent enables gentle and effective extraction of soluble protein from baculovirus-infected insect cells grown in suspension or monolayer culture. The baculovirus insect cell expression system is an efficient and popular system for production of recombinant (eukaryotic) proteins in cell culture. Proteins expressed in baculoviral systems can be used for structural analyses, biochemical assays and a variety of other applications. I-PER Reagent maintains functionality of extracted proteins and is directly compatible with downstream applications such as protein assays, Western blotting and 6xHis-tagged protein purification.
UOM: 1 * 250 mL
Product Image-786-PSB

Protein solubilisation buffer

Catalog Number: (786-PSB)
Supplier: G-Biosciences
Description: FOCUS™ protein solubilisation buffer is a dry, urea-based buffer for protein solubilisation. It is a pre-mixed formulation of urea, thiourea, CHAPS and non detergent sulphobetaine for maximum solubilising strength.
UOM: 1 * 50 mL
Product Image-PIER16189

Luciferase cell lysis buffer (2X), Pierce™

Catalog Number: (PIER16189)
Supplier: Thermo Scientific
Description: The Thermo Scientific Pierce luciferase cell lysis buffer effectively lyses adherent cells or pelleted suspension cells for analysis of promoter expression in luciferase assays.
UOM: 1 * 250 mL
Product Image-PIER87788

IP lysis buffer, Pierce™

Supplier: Thermo Scientific
Description: The IP lysis buffer is a mammalian whole cell lysis reagent based on a modified RIPA buffer formulation without SDS.

Product Image-PIER78991

Y-PER™ Yeast Protein Extraction Reagent

Supplier: Thermo Scientific
Description: This reagent uses a mild detergent lysis procedure to efficiently release functionally active solubilised proteins.

Product Image-786-411

Protein extraction kit, Insect PE LB™

Catalog Number: (786-411)
Supplier: G-Biosciences
Description: Insect PE LB™ has been developed for extraction of total biologically active, soluble proteins from adherent or suspension cultured insect cells, including Sf9 and Sf21. Insect PE LB™ utilises a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. The Insect PE LB™ is fully compatible with downstream processes, such as electrophoresis and chromatography. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Insect PE LB™.
UOM: 1 * 250 mL
Product Image-87790.

Subcellular protein fractionation kit for tissues

Supplier: Thermo Scientific
Description: This kit is ideal for cell fractionation and enrichment of proteins from five different cellular compartments within two hours.

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Genomic DNA extraction buffers

Supplier: OMEGA BIO-TEK
Description: P2 buffer, Omega Bio-Tek

Product Image-786-233

Extraction buffers, FOCUS™

Supplier: G-Biosciences
Description: FOCUS™ Extraction Buffers are experimentally optimised concentrations of critical agents, buffering and stabilising agents, including urea, thiourea, Nonidet® P-40, CHAPS, and sulphobetaines (SB). They are designed to produce optimal protein extraction and improved spot resolution for 2D gel analysis.

Product Image-PIER78505

Mammalian protein extract reagents, M-PER™

Supplier: Thermo Scientific
Description: The M-PER Mammalian Protein Extraction Reagent is designed to provide highly efficient total protein extraction from cultured mammalian cells.

Product Image-PIER78835

Nuclear and cytoplasmic extraction reagents, NE-PER

Supplier: Thermo Scientific
Description: The NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours. The nuclear protein extraction method involves simple, stepwise lysis of cells and centrifugal isolation of nuclear and cytoplasmic protein fractions. A benchtop microcentrifuge, tubes and pipettors are the only tools required. The NE-PER Reagents efficiently solubilise and separate cytoplasmic and nuclear proteins into fractions with minimal cross-contamination or interference from genomic DNA and mRNA. The isolated proteins can be used to perform immunoassays as well as protein interaction studies such as mobility shift assays (EMSA), co-immunoprecipitation (Co-IP) and pull-down assays.

Image Unavailable-70923-4

Protein extraction reagent, BugBuster® (primary amine-free), Millipore®

Supplier: Merck Millipore (Novagen)
Description: BugBuster® (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilisation or cross-linking. The PIPPS buffer used in BugBuster® (primary amine-free) has a similar buffer capacity and pH range as the original Tris-buffered BugBuster®, but will not complex metal ions, also making it ideally suited for extraction of metal dependent proteins.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
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