You Searched For: Protein Extraction

Supplier: G-Biosciences

Description: Antibody Fab and F(ab)₂ Fragmentation of Mouse IgG₁ Kits are designed for the generation and isolation of Fab and F(ab)₂ fragments from mouse IgG₁ molecules. The kit utilises Immobilised Ficin resin. Ficin (or Ficain) (~25000 Da) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge region. Ficin has an effective range of pH 4 to 9,5 with an optimal pH of 6,5 and cleaves bonds that involve uncharged or aromatic amino acids. Ficin is typically used to cleave mouse IgG₁ as this is difficult to cleave with papain and pepsin. In the presence of 1 to 4 mM or 10 to 20 mM cysteine, ficin generates F(ab)₂ and Fab fragments respectively.

Catalog Number: (786-681)

Supplier: G-Biosciences

Description: InGel™ Blue provides a complete set of reagents for the in-gel tryptic digestion and extraction of peptides for mass spectrometry (MALDI and LC MS/MS). The kit is specifically designed for use with Coomassie® or fluorescent stained protein spots/bands.

UOM: 1 * 1 KIT

Catalog Number: (DGA01)

Supplier: G-Biosciences

Description: The 'critical micelle concentration' (CMC) of a detergent varies with temperature, pH, ionic strength, detergent concentration, purity and presence of organic agents in the detergent. Using a large excess of detergent may pose problems during purification procedures or other downstream applications.

UOM: 1 * 500 items

Catalog Number: (786-793)

Supplier: G-Biosciences

Description: Ficin (or Ficain) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex is that has the endopeptidase activity to cleave Immunoglobulin G (IgG) molecules in the hinge region.

UOM: 1 * 5 mL

Catalog Number: (786-598)

Supplier: G-Biosciences

Description: Immobilised biotin resin is designed for the high affinity chromatography purifications of avidin, streptavidin and neutravidin protein. The resin consists of biotin coupled to 6% cross-linked agarose.

UOM:

Catalog Number: (786-249)

Supplier: G-Biosciences

Description: FOCUS™ Membrane Proteins for the fractionation of highly enriched membrane proteins.

UOM: 1 * 1 KIT

Catalog Number: (786-257)

Supplier: G-Biosciences

Description: Specifically designed for yeast research and supplied with yeast specific reagents. Extracts and solubilises nearly all of the proteins from yeast, including membrane and soluble proteins. Extraction is based on gentle lysis of yeast cells with a yeast lytic enzyme preparation, LongLife™ Zymolyase®, which has improved stability and shelf life. Enzymatic action is followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilises even the most difficult proteins.

UOM: 1 * 1 KIT

Catalog Number: (786-244)

Supplier: G-Biosciences

Description: SilverOUT removes interfering silver ions from gel pieces.

UOM: 1 * 100 Tests

Supplier: Thermo Scientific

Description: B-PER® Bacterial Protein Extraction Reagents are designed to extract soluble protein from bacterial cells. The B-PER® Bacterial Protein Extraction Kit eliminates the need for harsh mechanical procedures, like sonication. The easy-to-use cell lysis solution uses mild non-ionic detergents to disrupt cells and solubilise proteins without denaturation. Enzyme supplements such as DNase I and Lysozyme allow for improved yield of large molecular weight proteins which are difficult to purify. B-PER® is also compatible with downstream protein purification methods such as GST purification and His-tagged immobilised metal affinity chromatography (IMAC).

Catalog Number: (786-247)

Supplier: G-Biosciences

Description: FOCUS™ Soluble & Insoluble is a complete kit for the selective preparation of soluble (hydrophilic) and insoluble (hydrophobic) proteins from mammalian tissues and cells, plants, yeast, bacteria, and other biological samples. The kit comes with reagents necessary for fractionation of soluble and insoluble fractions, including a strong chaotropic extraction buffer to solubilise difficult proteins.

UOM: 1 * 1 KIT

Catalog Number: (786-253)

Supplier: G-Biosciences

Description: FOCUS™ Glycoprotein kit is based on lectin binding of specific glycoproteins with terminal α-D mannosyl and α-D glycosyl proteins.

UOM: 1 * 1 KIT

Supplier: G-Biosciences

Description: The use of peptides for the generation of antibodies against specific peptides has become an essential tool in proteomic research. A crucial step in using peptides as antigens is their coupling to carrier proteins. Peptides are designed to be good epitopes by themselves; however they fail to elicit a strong antibody response. The reason for this is that the epitopes are able to bind to the B-cells, but are too small to contain additional motifs required to associate with class II molecules and T-cell receptors. The carrier proteins, once coupled to the peptides, provide these additional motifs and generate a strong antibody response. All three carrier proteins are also offered as activated carrier proteins, named as ActiveHOOK™.

Supplier: G-Biosciences

Description: DetergentOUT™ Tween® is a spin column format detergent removal resin for polysorbate or Tween® detergents or surfactants.

Catalog Number: (786-018)

Supplier: G-Biosciences

Description: FOCUS™ Global Fractionation kit is designed to fractionate complex biological samples into cytosolic and membrane fractions. The resulting membrane fractions are subsequently fractionated into either peripheral and integral membrane proteins or lipid raft associated proteins and detergent soluble membrane proteins.

UOM: 1 * 1 KIT

Catalog Number: (PIER88279)

Supplier: Thermo Scientific

Description: The Pierce™ Mouse Embryonic Fibroblast (MEF) Isolation Kit enables high yield isolation and culture of viable primary fibroblasts from mouse embryos.

UOM: 1 * 1 KIT

Catalog Number: (786-246)

Supplier: G-Biosciences

Description: Extracts and solubilises nearly all of the proteins from mammalian samples, including membrane as well as soluble proteins, by a strong chaotropic extraction buffer to solubilise even the most difficult proteins. Suitable for biological samples from animal tissues and adherent and suspension cells.

UOM: 1 * 1 KIT