You Searched For: Protein Linking and Labeling Systems

Catalog Number: (PIER21121)

Supplier: Thermo Scientific

Description: Pierce™ Avidin is used in biotin-based detection methods, including Western blotting or ELISA detection with appropriate enzyme substrates. It is a purified protein from egg whites and it is stable over wide range of pH and temperature.

UOM: 1 * 10 mg

Supplier: Thermo Scientific

Description: A common target for protein modification is the carbohydrate portion of glycoproteins, which can be reacted with hydrazide derivatives of biotin. Oxidative treatment of glycoproteins using 10 mM periodate is used to generate reactive aldehydes from the cis-diols of a variety of carbohydrate moieties.

Supplier: Thermo Scientific

Description: DyLight™ Fluor phosphine reagents are phosphine-activated fluorescent dyes for specific labeling and detection of azide-tagged molecules, which enables use of fluorescence imaging in metabolic labeling strategies.

Supplier: G-Biosciences

Description: HOOK™ Biotin-PEG₂-Amine, and its long chain form, HOOK™ Biotin-PEG₃-Amine, are carboxyl reactive biotinylation reagents. These agents contain terminal amines and react with carboxyl groups found at the carboxyl termini, aspartate, and glutamate side chains. The reaction is mediated by a water-soluble carbodiimide (EDC). The carbodiimide activates the carboxyl group and reacts with the amines (-NH₂) on the biotinylation agent to form an amide bond. This reaction is rapid and takes just a few minutes to complete. Under these conditions, hydrazide-derivatives of biotin reagents may also react with the carboxyls.

Catalog Number: (BG-20)

Supplier: G-Biosciences

Description: Photoreactive agents, on exposure to ultraviolet light, become active and bind non specifically with neighbouring molecules. These reagents are suitable for labelling molecules that do not contain easily reactable functional groups. Photoreactive reagent HOOK™ Psoralen-PEO-Biotin reacts with and labels nucleic acids and protein molecules. When reacted with nucleic acids, it crosslinks with pyrimidine bases. Crosslinking does not interfere with hybridisation applications.

UOM: 1 * 5 mg

Supplier: G-Biosciences

Description: The HOOK™ Dye Labeling Kit (TAMRA-SE (5-(and-6)- Carboxytetramethylrhodamine succinimidyl ester, mixed isomers) is based on tetramethylrhodamine, one of the most common fluorophores used in the labelling of peptides, proteins, nucleic acids and nucleotides. It absorbs green visible light at 546 nm and emits an orange-red visible light at a maximum emission of 575 nm.

Supplier: Thermo Scientific

Description: Pierce™ azide-labeled sugars provide a highly specific approach for studying glycoproteins through <i>in vivo</i> metabolic labeling and chemoselective ligation.

Catalog Number: (PIER31488)

Supplier: Thermo Scientific

Description: The Thermo Scientific™ EZ-Link™ Plus Activated Peroxidase is an amine-reactive horseradish peroxidase (HRP) that provides higher conjugate yields (>95%) than glutaraldehyde chemistry.

UOM: 1 * 1 KIT

Supplier: Thermo Scientific

Description: The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.

Supplier: Thermo Scientific

Description: Maleimide Activated Horseradish Peroxidase is for preparation of HRP conjugates with proteins, peptides or other ligands that contain sulfhydryl groups, such as reduced cysteines.

Supplier: Thermo Scientific

Description: DyLight™ Dyes have absorption spectra ranging from 350 nm to 770 nm and match the output wavelengths of common fluorescence instrumentation.

Supplier: Thermo Scientific

Description: The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.

Catalog Number: (PIER20160)

Supplier: Thermo Scientific

Description: Pierce™ RNA 3' End Biotinylation Kit is optimised for labeling the 3' terminal end of RNA probes to facilitate their use as probes or targets in EMSA and other methods for studying protein-RNA interactions.

UOM: 1 * 1 KIT

Supplier: Thermo Scientific

Description: Pierce™ HRP conjugated avidin is used in biotin-based detection methods, including Western blotting or ELISA detection with appropriate enzyme substrates.

Supplier: Thermo Scientific

Description: Cross linkers are used for the identification of near-neighbor protein relationships and ligand-receptor interactions.

Catalog Number: (PIER46610)

Supplier: Thermo Scientific

Description: Pierce™ Fluorescence Biotin Quantitation Kit requires only 10 μl of sample to accurately measure the biotinylation level of labeled antibodies and other biomolecules.

UOM: 1 * 1 KIT