Print…

You Searched For: Sanger Sequencing Reagents

Sequencing reagents facilitate the processes when determining the precise order of nucleotides within DNA strands through sorting the fragments based on size. Enzymes are optimized to work together for high-efficiency removal of unincorporated primers and nucleotides. These reagents produce exceptional sequencing results from difficult templates, such as those with high GC content and secondary structures. Essential for basic and applied research procedures, sequencing reagents allow researchers to discover genotypes and phenotypes of specimen.

Sequencing reagents facilitate the processes when determining the precise order of nucleotides within DNA strands through sorting the fragments based on size. Enzymes are optimized to work together for high-efficiency removal of unincorporated primers and nucleotides. These reagents produce exceptional sequencing results from difficult templates, such as those with high GC content and secondary structures. Essential for basic and applied research procedures, sequencing reagents allow researchers to discover genotypes and phenotypes of specimen.


6  results were found

Refine Results Sort by
SearchResultCount:"6"

Sort Results

List View Easy View (new)

Rate These Search Results

Product Image-MAG-FRAG-I-5

Fragment selection kits, Axygen® AxyPrep™ FragmentSelect-I Kit

Catalog Number: (MAG-FRAG-I-5)
Supplier: Corning
Description: The AxyPrep™ FragmentSelect Kits utilise a paramagnetic bead technology for quick high throughput optimised DNA size selection suitable for various next generation sequencing platforms. The AxyPrep™ FragmentSelect-I kit is optimised for Illumina next generation sequencing platforms and Life Technologies (SOLiD™) fragment size selection needs simplifying the library construction workflow.
UOM: 1 * 1 KIT
Image Unavailable-786-15

Glutamic-C™, sequencing grade

Catalog Number: (786-15)
Supplier: G-Biosciences
Description: SG-Glutamic-C™ is a serine endopeptidase, from<i> S. aureus V8</i>, that is highly specific for the cleavage of peptide bonds at the carboxy side of either aspartic or glutamic acid, depending on the buffer used. In Tris-HCl buffer, in particular in the absence of phosphate ions, the enzyme is specific for the glutamyl site. Recommended buffers for fragmentation of proteins using this enzyme are 50 mM Tris-HCI, pH 8,0 or bicarbonate buffer. Highly purified preparations of SG-Glutamic-C™ are chemically modified making the enzyme both resistant to autolysis and stabilises its enzymatic activity.
UOM: 1 * 2 items
Image Unavailable-786-14

Lysine-C™, sequencing grade

Catalog Number: (786-14)
Supplier: G-Biosciences
Description: SG-Lysine-C™ endopeptidase, from <i>Lysobacter enzymogenes</i>, is a serine protease highly specific in cleaving peptide bonds at the carboxy side of lysine. Highly purified preparations of SG-Lysine-C™ are chemically modified making the enzyme resistant to autolysis and stabilising its enzymatic activity.
UOM: 1 * 2 items
Image Unavailable-786-11

Arginine-C™, sequencing grade

Catalog Number: (786-11)
Supplier: G-Biosciences
Description: SG-Arginine-C™ endopeptidase (Clostripain, from <i>C. histolyticum</i>) specifically hydrolyses the carboxy peptide bond of arginine. SG-Arginine-C™ has been modified chemically by a propriety process to render the enzyme resistant to autolysis and stabilise enzymatic activity. In addition, as a sulfhydryl enzyme, SG-Arginine-C™ is susceptible to inactivation by oxidation and as a result requires reducing agents for protection. The enzyme also requires calcium ion for maximal activity. A special reconstitution buffer is supplied, which contains reducing agents and activators to maintain enzyme activity.
UOM: 1 * 2 items
Image Unavailable-786-13

Chymotrypsin™, sequencing grade

Catalog Number: (786-13)
Supplier: G-Biosciences
Description: SG-Chymotrypsin™ is a serine endopeptidase, which predominantly cleaves peptide bonds on the carboxy side of tyrosine, phenylalanine and tryptophan. In addition, chymotrypsin has a low catalytic activity against the carboxy side of leucine, methionine, alanine, aspartic and glutamic acids. It is therefore recommended to always use the shortest digestion time possible.
UOM: 1 * 2 items
Product Image-25-9005-98

Sequencing reaction purification, illustra™ AutoScreen 96 Well Plates

Catalog Number: (25-9005-98)
Supplier: Cytiva
Description: AutoScreen 96 well plate consists of a 96-well filter plate containing DNA grade Sephadex™ G-50 for purification of sequencing reactions prior to analysis on MegaBACE 1000 and can be used for other size exclusion applications.
UOM: 1 * 10 items
Inquire for Price
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 88 22222.
1 - 6 of 6
no targeter for Bottom