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Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.

Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.


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Protein extraction reagent, BugBuster® HT, Millipore®

Supplier: Merck Millipore (Novagen)
Description: BugBuster® HT Protein Extraction Reagent combines BugBuster® Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster® HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from <i>E. coli</i> without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.

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HEPES lysis buffer with NP-40 (2X)

Catalog Number: (J63867.AK)
Supplier: Alfa Aesar
Description: HEPES lysis buffer with NP-40 (2X), Thermo Fisher Scientific
UOM: 1 * 250 mL
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NP-40 lysis buffer, low salt

Supplier: Alfa Aesar
Description: NP-40 lysis buffer, low salt, Thermo Fisher Scientific

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GTE buffer

Supplier: Alfa Aesar
Description: Thermo Fisher Scientific

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Succinate, 0,2 M buffer solution, pH 5,0

Supplier: Alfa Aesar
Description: Succinate, 0.2M buffer soln., pH 5.0, Thermo Fisher Scientific

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Triton® X-100 lysis buffer with glycerol (2X), pH 7,4

Catalog Number: (J63866.AK)
Supplier: Alfa Aesar
Description: Triton® X-100 lysis buffer with glycerol (2X), pH 7.4, Thermo Fisher Scientific
UOM: 1 * 250 mL
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RIPA buffer with Triton® X-100 (5X)

Supplier: Alfa Aesar
Description: RIPA buffer with Triton® X-100 (5X), Thermo Fisher Scientific

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MES lysis buffer with Triton® X-100

Catalog Number: (J62693.AK)
Supplier: Alfa Aesar
Description: MES lysis buffer with Triton® X-100, Thermo Fisher Scientific
UOM: 1 * 250 mL
Product Image-786-649

Lysis buffers, red blood cells

Supplier: G-Biosciences
Description: These DNase- and RNase-free buffers and reagents are designed for high quality research.

MSDS

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ACK Lysing Buffer (1x), TheraPEAK™

Catalog Number: (LONZBP10-548E)
Supplier: LONZA
Description: Ammonium-Chloride-Potassium (ACK) Lysing Buffer is used to lyse red blood cells in preparations containing white blood cells.
UOM: 1 * 100 mL

MSDS


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PIPES lysis buffer with NP-40 (2X)

Catalog Number: (J60568.AK)
Supplier: Alfa Aesar
Description: PIPES lysis buffer with NP-40 (2X), Thermo Fisher Scientific
UOM: 1 * 250 mL
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HEPES protein extraction buffer (5X)

Catalog Number: (J63297.AK)
Supplier: Alfa Aesar
Description: Thermo Fisher Scientific
UOM: 1 * 250 mL

MSDS Certificates


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NP-40 lysis buffer (2X)

Catalog Number: (J62805.AK)
Supplier: Alfa Aesar
Description: NP-40 lysis buffer (2X), Thermo Fisher Scientific
UOM: 1 * 250 mL
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Protein extraction reagent, BugBuster® 10X, Millipore®

Supplier: Merck Millipore (Novagen)
Description: BugBuster® 10X is a concentrated formulation of the proprietary detergents employed in BugBuster® without the addition of salts or buffer components. Concentrated BugBuster® provides a flexible alternative to the ready-to-use standard 1X BugBuster®, allowing user-defined dilution and addition of buffer components. BugBuster® 10X has all of the bioprocessing benefits of standard BugBuster® plus the freedom to control pH, reagent concentration, and buffer additives necessary for maximum extraction and activity of your target protein.

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E. coli lysis buffer

Catalog Number: (J63402.AK)
Supplier: Alfa Aesar
Description: E. coli lysis buffer, Thermo Fisher Scientific
UOM: 1 * 250 mL

MSDS Certificates


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RIPA Lysis and Extraction Buffer

Supplier: G-Biosciences
Description: A complete lysis buffer for the release of cytoplasmic, membrane and nuclear proteins from adherent and suspension cultured mammalian cells. The RIPA lysis buffer is fully compatible with many applications, including reporter assays, protein assays, immunoassays and other protein purification techniques.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
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This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 88 22222.
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