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You Searched For: Western Blotting Reagents

Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.

Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.


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Product Image-PIER34000

CN/DAB substrate kit, Pierce™

Catalog Number: (PIER34000)
Supplier: Thermo Scientific
Description: Pierce™ CN/DAB Substrate Kit is for chromogenic detection of horseradish peroxidase in immunohistochemistry and immunoblotting applications. The formed product is a black precipitate that photographs well and is useful in double staining applications. The coloured product is soluble in xylenes but not soluble in aqueous buffer such as Tris or phosphate. This combination of DAB and CN has a synergistic effect that results in greater sensitivity than either reagent alone.
UOM: 1 * 1 KIT
Product Image-PIER34042

Substrate for western blotting, 1-Step™ NBT/BCIP

Catalog Number: (PIER34042)
Supplier: Thermo Scientific
Description: The 1-Step NBT/BCIP is a one-component sensitive precipitating alkaline phosphatase substrate for immunoblotting, immunohistochemistry, and <i>in situ</i> hybridisation applications.
UOM: 1 * 250 mL
Product Image-PIER34018

1-Step™, Pierce™ TMB-Blotting substrate solution

Catalog Number: (PIER34018)
Supplier: Thermo Scientific
Description: The 1-Step TMB-Blotting is a one component horseradish peroxidase (HRP) substrate for Western blotting and immunohistochemistry. TMB is highly sensitive and generates a blue-purple precipitate. This substrate is provided ready to use for maximum convenience.
UOM: 1 * 250 mL
Image Unavailable-PIER15162

NS/K Fluorogenic substrate kit, QuantaBlu™, Pierce™

Catalog Number: (PIER15162)
Supplier: Thermo Scientific
Description: QuantaBlu™ NS/K Fluorogenic Substrate Kit is a two-component kit containing proprietary fluorescent substrate and peroxide solutions for non-stopped kinetic assays.
UOM: 1 * 1 KIT
Image Unavailable-786-423

High molecular weight transfer buffer (5X)

Catalog Number: (786-423)
Supplier: G-Biosciences
Description: High molecular weight transfer buffer (5X) is designed to facilitate the transfer of notoriously difficult high molecular weight proteins (>70 kDa) during Western blotting.
UOM: 1 * 1 L
Product Image-PIER31490

Horseradish peroxidase, Pierce™

Catalog Number: (PIER31490)
Supplier: Thermo Scientific
Description: Pierce™ Horseradish peroxidase (HRP) is purified horseradish peroxidase enzyme for use in activity assays and conjugation to antibodies for ELISA, Western blot and immunohistochemistry applications.
UOM: 1 * 10 mg
Product Image-PIER34002

DAB substrate

Catalog Number: (PIER34002)
Supplier: Thermo Scientific
Description: DAB (3,3'-diaminodbenzidine) is a sensitive colorimetric substrate used with horseradish peroxidase (HRP) in immunohistochemistry and immunoblotting applications. HRP catalyses hydrogen peroxide oxidation of substrates such as DAB. Electrons are transferred by HRP from the DAB to the peroxide to yield an insoluble brown product.
UOM: 1 * 1 KIT
Product Image-PIER15165

HRP conjugates, HisProbe™

Catalog Number: (PIER15165)
Supplier: Thermo Scientific
Description: HisProbe™-HRP is a nickel (Ni²⁺)-activated derivative of horseradish peroxidase (HRP) that enables direct, IMAC-based detection of His-tagged proteins and other histidine-rich proteins in Western blots and microplates.
UOM: 1 * 2 mg
Product Image-PIER17075

Biotin random prime DNA labelling kit, North2South™

Catalog Number: (PIER17075)
Supplier: Thermo Scientific
Description: North2South™ Biotin Random Prime DNA Labeling Kit uses random heptanucleotides, Klenow fragment and biotin-nucleotides to produce biotinylated DNA templates for DNA hybridisation and detection methods.
UOM: 1 * 1 KIT
Product Image-PIER21230

Pierce™ Clean-Blot™ IP Detection Kit and Reagents

Supplier: Thermo Scientific
Description: Clean-Blot™ IP detection kit (HRP) contains enough Clean-Blot™ IP detection reagent and accessory reagents for 25 mini-blots or 2000 cm² of membrane. Clean-Blot™ IP detection reagent is a horseradish peroxidase conjugate that is optimised for post-immunoprecipitation western blot detection of primary antibodies without interference from denatured IP antibody fragments.

Product Image-PIER62299

Fluorescent western blot stripping buffer, Restore™

Supplier: Thermo Scientific
Description: Restore™ Fluorescent Western Blot Stripping Buffer is a gentle and highly effective reagent for quickly removing primary and near-infrared (IR) dye-labeled secondary antibodies from western blots.

Product Image-PIER90105

S-Nitrosylation western blot kit, Pierce™

Supplier: Thermo Scientific
Description: The Pierce™ S-Nitrosylation Western Blot Kit enables sensitive detection of protein S-nitrosocysteine post-translational modifications.

Product Image-PIER46641

Western blot enhancer, SuperSignal™, Pierce

Supplier: Thermo Scientific
Description: SuperSignal™ Western Blot Enhancer is a membrane treatment and a primary antibody diluent that has been shown to increase intensity of a target protein band on a Western blot and to enable detection of a target analyte at a level at which it could not be detected without enhancement.

Product Image-PIER15169

Fluorogenic peroxidase substrate kit, QuantaBlu™

Catalog Number: (PIER15169)
Supplier: Thermo Scientific
Description: QuantaBlu™ Fluorogenic peroxidase substrate kit is a three-component kit containing proprietary fluorescent substrate, peroxide and stop solutions for stopped (endpoint) or non-stopped assays.
UOM: 1 * 1 KIT

MSDS


Product Image-PIER89818

Pierce™ Biotin 3' End DNA Labelling Kit

Catalog Number: (PIER89818)
Supplier: Thermo Scientific
Description: This Biotin 3' End DNA Labelling Kit is used for tagging single-stranded DNA primers with biotin for use in non radioactive electrophoretic mobility shift assays (EMSA) and other nucleic acid detection methods.
UOM: 1 * 1 KIT
Product Image-PIER21050

Pierce™ Western Blot Signal Enhancer

Catalog Number: (PIER21050)
Supplier: Thermo Scientific
Description: Western blot signal enhancer is a membrane treatment that has been shown to increase intensity of a target protein band on a Western blot and to enable detection of a target analyte at a Level at which it could not be detected without enhancement.
UOM: 1 * 500 mL
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
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This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 88 22222.
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