Nile blue chloride (Basic blue 12) 85% (dye content), Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY

Synonyms: Nile Blue A chloride, 5-Amino-9-(diethylamino)benzo[a]phenoxazin-7-ium chloride

M-Clarity™ quality level = MQ200

222550-25G 222550-5G
222550-25GEA 340 EUR
222550-25G 222550-5G
Nile blue chloride (Basic blue 12) 85% (dye content), Sigma-Aldrich®
Nile blue chloride (Basic blue 12)

Nile blue chloride has been used to stain non-aqueous phase in fluorescence microscopy studies.


Nile blue chloride is a green colored powder and is a member of oxazine group of dyes. It changes its color from blue (pH 9,4) to purple-red (11,0). Nile blue chloride has been used to detect DNA and lipids. In addition, it also helps to identify bacterial growth. Nile blue chloride also facilitates diagnosis of disease linked with amyloid accumulation.

Formula: C₂₀H₂₀ClN₃O
MW: 353,85 g/mol
Storage Temperature: Room Temperature
MDL Number: MFCD00011936
CAS Number: 2381-85-3
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Specification Test Results

Appearance (Colour) Dark Green to Black
Appearance (Form) Powder
Infrared spectrum Conforms to Structure
Lambda Max 634 - 642 nm
Carbon Content 56.3 - 79.4%
Nitrogen Content 9.7 - 13.9%

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