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Imidazole, EMPROVE® EXPERT ACS

Supplier: MERCK PRODUCTION CHEMICALS
Description: Imidazole is a critical buffer substance for bioprocesses to enhance the purity of your target protein. Its most widely used application is the preparation of buffer solutions to operate downstream purification columns, packed with resins.

Product Image-786-133

Arrest™ extraction buffer

Catalog Number: (786-133)
Supplier: G-Biosciences
Description: Arrest™ Extraction Buffer is an optimised combination of various chaotropic agents and RNase inhibitors, which inhibits RNase rapidly. It may be used in conjunction with any RNA extraction method, including extractions based on phenol, chloroform and other organic solvents and detergents.
UOM: 1 * 200 Tests
Image Unavailable-69067-3

Enterokinase cleavage capture kit

Catalog Number: (69067-3)
Supplier: Merck Millipore (Novagen)
Description: The Enterokinase Cleavage Capture Kit is designed for highly specific cleavage of fusion proteins followed by rapid, affinity-based capture and removal of enterokinase. Following cleavage of the target protein, rEK is removed with >99% efficiency from the reaction by affinity capture on EKapture™ Agarose. Following capture of rEK, the EKapture Agarose is removed by spin filtration, because the same buffer conditions are used for both cleavage and capture no buffer changes are necessary.
UOM: 1 * 1 KIT
Product Image-PIER77720

TCEP solution, neutral pH, Bond-Breaker™ Pierce™

Catalog Number: (PIER77720)
Supplier: Thermo Scientific
Description: Bond-Breaker™ TCEP Solution (neutral pH) is a stable, 0,5 M solution of the thiol-free, phosphine-based TCEP compound, useful as a 10X stock for addition to SDS-PAGE sample loading buffers to reduce protein disulphide bonds.
UOM: 1 * 5 mL

MSDS


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Streptavidin agarose

Catalog Number: (69203-3)
Supplier: Merck Millipore (Novagen)
Description: Streptavidin agarose consists of cross-linked agarose covalently coupled with pure streptavidin under controlled conditions. The stable linkage to the resin minimizes leaching of the streptavidin while maintaining full binding activity. The matrix is suitable for use in column and batch formats for any application that requires high biotin binding capacity and low non-specific binding. The resin has no detectable protease, DNase, or RNase contamination.
UOM: 1 * 5 mL
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Sodium acetate, anhydrous 99,0, powder_crystalline ACS

Supplier: MP Biomedicals
Description: Sodium acetate is used as a buffer in the buffering range of pH 3.6-5.6. It is also used in the purification and precipitation of nucleic acids, protein crystallization, staining of gels in protein gel electrophoresis, HPLC, mordant dyeing, DNA microarray studies of E. coli response, investigation of protein unfolding during reverse phase chromatography.

Product Image-29-4233-83

Biacore consumables and accessories

Catalog Number: (29-4233-83)
Supplier: Cytiva
Description: Biacore maintenance kits are convenient kits for proper instrument maintenance.
UOM: 1 * 1 KIT
Product Image-PIER89802

I-PER™ Insect Cell Protein Extraction Reagents

Catalog Number: (PIER89802)
Supplier: Thermo Scientific
Description: The I-PER Insect Cell Protein Extraction Reagent enables gentle and effective extraction of soluble protein from baculovirus-infected insect cells grown in suspension or monolayer culture. The baculovirus insect cell expression system is an efficient and popular system for production of recombinant (eukaryotic) proteins in cell culture. Proteins expressed in baculoviral systems can be used for structural analyses, biochemical assays and a variety of other applications. I-PER Reagent maintains functionality of extracted proteins and is directly compatible with downstream applications such as protein assays, Western blotting and 6xHis-tagged protein purification.
UOM: 1 * 250 mL
Product Image-RPN3006

Amersham™ ECL Gold Hybridization Buffer, Cytiva

Catalog Number: (RPN3006)
Supplier: Cytiva
Description: ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.
UOM: 1 * 1 items
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Accessories for Fast DNA™ and Fast DNA™ SPIN Kits, MP Biomedicals

Supplier: MP Biomedicals
Description: Nucleic Acid Purification Kits and Reagents, Sodium phosphate buffer

MSDS

Product Image-27-5340-01

Dye terminator removal, illustra™ AutoSeq™ G-50 columns

Supplier: Cytiva
Description: illustra™ AutoSeq™ G-50 columns are specifically designed to remove fluorescent dye-terminators from sequencing reactions prior to analysis on automated sequencers. Effective purification is essential for high quality sequencing results since residual dye-terminators can obscure data. illustra™ AutoSeq™ G-50 consists of MicroSpin™ columns containing Sephadex™ G-50, pre-equilibrated in double-distilled water. This is important because even the small amount of salt in traditional buffers (such as TE buffer) can cause electrophoretic artifacts on salt-sensitive automated sequencers.

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Protein extraction reagents, BugBuster® and BugBuster® plus Benzonase®, Millipore®

Supplier: Merck Millipore (Novagen)
Description: BugBuster® Protein Extraction reagent is formulated for the gentle disruption of the cell wall of <i>E. coli</i>, liberating soluble protein. It provides a simple, rapid, low-cost alternative to mechanical methods such as French Press or sonication for releasing expressed target protein in preparation for purification or other applications. The formulation utilises a detergent mix that is capable of cell wall perforation without denaturing soluble protein.

Product Image-PIER90084

Bacterial Protein Extraction Kits, B-PER™

Supplier: Thermo Scientific
Description: B-PER® Bacterial Protein Extraction Reagents are designed to extract soluble protein from bacterial cells. The B-PER® Bacterial Protein Extraction Kit eliminates the need for harsh mechanical procedures, like sonication. The easy-to-use cell lysis solution uses mild non-ionic detergents to disrupt cells and solubilise proteins without denaturation. Enzyme supplements such as DNase I and Lysozyme allow for improved yield of large molecular weight proteins which are difficult to purify. B-PER® is also compatible with downstream protein purification methods such as GST purification and His-tagged immobilised metal affinity chromatography (IMAC).

Product Image-786-316

Cell surface protein isolation and labelling kit, HOOK™

Catalog Number: (786-316)
Supplier: G-Biosciences
Description: The HOOK™ Cell Surface Protein Isolation Kit uses HOOK™ biotin labelling and purification technology in conjunction with Mammalian Cell PE LB™ Lysis Buffer to conveniently label cell surface proteins and isolate them for further analysis, including Western blotting. The Kit is supplied with all the necessary reagents and buffers for convenience and improved reproducibility. The kit is compatible with a wide variety of mammalian cells and can be used to compare treated and untreated cells and differences between different cell lines.
UOM: 1 * 1 KIT
Product Image-216-1098

Vacuum aspiration systems with trap flask

Catalog Number: (216-1098)
Supplier: GRANT INSTRUMENTS
Description: All in one system with integrated pump designed for routine aspiration of the supernatent alcohol/buffer from the walls of microtubes during DNA/RNA purification and other macromolecule reprecipitation techniques. An ideal personal tool for independent operation away from an in-line lab vacuum supply.
UOM: 1 * 1 items
Product Image-17-1128-01

Affinity chromatography kit, MAbTrap™

Catalog Number: (17-1128-01)
Supplier: Cytiva
Description: MAbTrap™ is an affinity chromatography kit for fast and effective purification of monoclonal and polyclonal IgG from ascites fluid, serum and cell culture supernatants. MAbTrap™ Kit contains everything necessary to perform a complete one step purification without using complicated equipment. It also eliminates time consuming buffer preparation and column packing.
UOM: 1 * 1 KIT
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
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