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Description: Amersham™ Protran® membranes are manufactured from 100% pure nitrocellulose to ensure the highest binding capacity possible. They are compatible with a variety of detection methods, including isotopic, chemiluminescent, colorimetric and fluorescent. No methanol pre-wetting step is required, making this the membrane of choice for proteins which prefer aqueous environments. Prior to transfer the membrane is simply wetted with water, and then placed in the transfer buffer. No other pretreatment steps are necessary.

Catalog Number: 10600045.

UOM: 1 * 25 items

Supplier: Cytiva

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Description: TempliPhi™ Kits efficiently prepare micrograms of circular DNA from picogram input material. The DNA templates are prepared by rolling circle amplification (RCA) using bacteriophage Phi29 DNA polymerase. TempliPhi™ uses an isothermal method for the exponential amplification of circular DNA. Phi29 DNA polymerase is active at 30 °C, enabling amplification to be performed at this temperature without the need for thermal cycling. The TempliPhi™ protocol requires less than 20 minutes of hands-on time to amplify 96 samples from bacterial colonies.

Catalog Number: 25-6400-50

UOM: 1 * 1 KIT

Supplier: Cytiva

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Description: HiTrap TALON® crude 1 and 5 ml columns allow for rapid separations and easy scale-up with a minimum of sample preparation and equipment.

Catalog Number: 29-0485-65

UOM: 1 * 1 items

Supplier: Cytiva

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Description: PD MiniTrap G-25 columns are prepacked, single-use gravity columns for desalting, buffer exchange, and sample cleanup.

Catalog Number: 28-9180-07

UOM: 1 * 50 items

Supplier: Cytiva

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Description: Superdex prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component

Catalog Number: 28-9893-34

UOM: 1 * 1 items

Supplier: Cytiva

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Description: NHS-activated Sepharose™ 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups. The resin provides a spacer arm to the coupled ligand and suitable for immobilising smaller protein and peptide ligands.

Catalog Number: 17-0906-01

UOM: 1 * 25 mL

Supplier: Cytiva

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Description: CNBr-activated Sepharose™ 4B is pre-activated media used for coupling antibodies or other large proteins containing -NH₂ groups to the Sepharose™ media, by the cyanogen bromide method, without an intermediate spacer arm.

Catalog Number: 17-0430-01

UOM: 1 * 15 g

Supplier: Cytiva

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Description: PD SpinTrap G-25 is a single-use column for cleanup of large biomolecules.

Catalog Number: 28-9180-04

UOM: 1 * 50 items

Supplier: Cytiva

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Description: Protein G Sepharose 4 Fast Flow has recombinant protein G immobilized by the cyanogen bromide (CNBr) method to Sepharose 4 Fast Flow.

Catalog Number: 17-0618-05

UOM: 1 * 200 mL

Supplier: Cytiva

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Description: Vivaspin® sample concentrators are designed for fast, nondenaturing concentration of biological samples by membrane ultrafiltration.

Catalog Number: 28-9323-61

UOM: 1 * 12 items

Supplier: Cytiva

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Description: PD-10 Columns are economical, plastic columns for gravity flow purification.

Catalog Number: 17-0435-01

UOM: 1 * 50 items

Supplier: Cytiva

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Description: Amersham™ Protran® Supported is a robust protein blotting membrane, with 0,2 µm pore size, made of reinforced nitrocellulose for multiple reprobings.

Catalog Number: 10600051.

UOM: 1 * 25 items

Supplier: Cytiva

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Description: HiTrap™ SP FF is pre-packed with SP Sepharose™ Fast Flow, and is a strong cation exchanger for small scale protein purifications as well as screening of binding and elutions conditions.

Catalog Number: 17-5054-01

UOM: 1 * 5 items

Supplier: Cytiva

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Description: HiTrap™ Protein G HP affinity columns are pre-packed with 1 or 5 ml of Protein G Sepharose™ high performance for purification and isolation of monoclonal and polyclonal IgG from ascites, serum and cell culture supernatants.

Catalog Number: 29-0485-81

UOM: 1 * 1 items

Supplier: Cytiva

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Description: HisTrap HP prepacked columns provide a fast, convenient and reproducible format for preparative purification of histidine-tagged (His-tag) recombinant proteins.

Catalog Number: 29-0510-21

UOM: 1 * 1 items

Supplier: Cytiva

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Description: Sephadex™ G-50 Medium is well established gel filtration medium for desalting and buffer exchange of biomolecules >30000 molecular weight. The larger bead size of G-50 Medium is suited for large scale separations.

Catalog Number: 17-0042-01

UOM: 1 * 100 g

Supplier: Cytiva

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