You Searched For: Dithiothreitol+(DTT,+Cleland\\\'s+reagent)

Catalog Number: (598250-1)

Supplier: Merck Millipore (Calbiochem‎)

Description: A water-soluble, neutral, and odorless disulfide reducing agent that is more stable and effective than DTT. THP is comparable with TCEP as a reducing agent. Its reduction capacity against oxidised glutathione is 0,232 meq/g (TCEP has a reduction capacity of 0,182 meq/g). THP is also more resistant to air oxidation as compared to DTT.

UOM: 1 * 1 g

Catalog Number: (RCH-003)

Supplier: G-Biosciences

Description: Recombinant human caspases 1 - 3 and 6 - 10 are supplied lyophilised in quantities of 25 units. One unit of the specific recombinant caspase is the enzyme activity that cleaves one nanomole of a specific caspase substrate per hour at 37 °C in a reaction solution containing 50mM HEPES (at a pH of 7,2), 50 mM NaCl, 0,1% CHAPS, 10 mM EDTA, 5% glycerol and 10 mM DTT.

UOM: 1 * 25 EU

Catalog Number: (RCH-009)

Supplier: G-Biosciences

Description: Recombinant human caspases 1 - 3 and 6 - 10 are supplied lyophilised in quantities of 25 units. One unit of the specific recombinant caspase is the enzyme activity that cleaves one nanomole of a specific caspase substrate per hour at 37 °C in a reaction solution containing 50mM HEPES (at a pH of 7,2), 50 mM NaCl, 0,1% CHAPS, 10 mM EDTA, 5% glycerol and 10 mM DTT.

UOM: 1 * 25 EU

Catalog Number: (RCH-010)

Supplier: G-Biosciences

Description: Recombinant human caspases 1 - 3 and 6 - 10 are supplied lyophilised in quantities of 25 units. One unit of the specific recombinant caspase is the enzyme activity that cleaves one nanomole of a specific caspase substrate per hour at 37 °C in a reaction solution containing 50mM HEPES (at a pH of 7,2), 50 mM NaCl, 0,1% CHAPS, 10 mM EDTA, 5% glycerol and 10 mM DTT.

UOM: 1 * 25 EU

Catalog Number: (RCH-001)

Supplier: G-Biosciences

Description: Recombinant human caspases 1 - 3 and 6 - 10 are supplied lyophilised in quantities of 25 units. One unit of the specific recombinant caspase is the enzyme activity that cleaves one nanomole of a specific caspase substrate per hour at 37 °C in a reaction solution containing 50mM HEPES (at a pH of 7,2), 50 mM NaCl, 0,1% CHAPS, 10 mM EDTA, 5% glycerol and 10 mM DTT.

UOM: 1 * 25 AU

Catalog Number: (RCH-007)

Supplier: G-Biosciences

Description: Recombinant human caspases 1 - 3 and 6 - 10 are supplied lyophilised in quantities of 25 units. One unit of the specific recombinant caspase is the enzyme activity that cleaves one nanomole of a specific caspase substrate per hour at 37 °C in a reaction solution containing 50mM HEPES (at a pH of 7,2), 50 mM NaCl, 0,1% CHAPS, 10 mM EDTA, 5% glycerol and 10 mM DTT.

UOM: 1 * 25 EU

Catalog Number: (RCH-002)

Supplier: G-Biosciences

Description: Recombinant human caspases 1 - 3 and 6 - 10 are supplied lyophilised in quantities of 25 units. One unit of the specific recombinant caspase is the enzyme activity that cleaves one nanomole of a specific caspase substrate per hour at 37 °C in a reaction solution containing 50mM HEPES (at a pH of 7,2), 50 mM NaCl, 0,1% CHAPS, 10 mM EDTA, 5% glycerol and 10 mM DTT.

UOM: 1 * 25 EU

Supplier: MP Biomedicals

Description: Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C

Catalog Number: (1.02035.0100)

Supplier: Merck

Description: Dragendorff's reagent, spray reagent for TLC, Supelco®

UOM: 1 * 100 mL

Catalog Number: (44578-100ML-F)

Supplier: Merck

Description: This non-aqueous Thiess, Reuther and Vagujfalvi variant of the reagent is used to detect alkaloids and other nitrogen compounds in TLC.

UOM: 1 * 100 mL

Catalog Number: (3901-10VL)

Supplier: SIGMA ALDRICH MICROSCOPY

Description: It contains p-Phenylenediamine diHCL (1 part) and catechol (2 parts). Commonly referred to as Hanker Yates reagent. Kit component in the Sigma Leukocyte peroxidase (myeloperoxidase) Procedure, 390A.

UOM: 1 * 10 items

Supplier: Merck

Description: Determination of ammonium/nitrogen salts using mercury containing materials.

MSDS

Catalog Number: (191844W)

Supplier: VWR Chemicals

Description: Vanadomolybdate reagent

UOM: 1 * 1 L

Catalog Number: (1.09293.0100)

Supplier: Merck

Description: Kovac's indole reagent for microbiology pH <1 (H₂O, 20 °C), Millipore®

UOM: 1 * 100 mL

Catalog Number: (4465962-1KT)

Supplier: Merck

Description: Keto Reagent Kit, Amplifex™, Supelco®

UOM: 1 * 1 KIT

Supplier: Merck

Description: Sanger's reagent ≥99.0% (by GC), LiChropur™ derivatisation reagent, for HPLC, Supelco®