You Searched For: Electrophoresis+Reagents

Supplier: Thermo Scientific

Description: Although optimising assay conditions is the best way to achieve optimum results, re-performing the gel electrophoresis process to test each new primary antibody or antibody concentration is time-consuming and expensive. Restore™ Western blot stripping buffer offers an effective alternative.

Catalog Number: (A9555.1000)

Supplier: PanReac AppliChem

Description: DNA-Dye NonTox is a highly sensitive, non toxic, fluorescent staining reagent for the detection of double-stranded DNA (dsDNA). The dye produces instant visualisation of DNA bands on gels upon blue light or UV illumination.

UOM: 1 * 1 mL

MSDS

Supplier: Alfa Aesar

Description: 1,4-Dithioerythritol 99% for electrophoresis

MSDS Certificates

Catalog Number: (PIER24614)

Supplier: Thermo Scientific

Description: Pierce silver stain rescue reagent enables removal of undesirable background from unevenly and overly developed silver-stained polyacrylamide electrophoresis gels.

UOM: 1 * 40 mL

Catalog Number: (EDVO203)

Supplier: EDVOTEK

Description: Isolation of high molecular weight chromosomal DNA is the first step in molecular cloning since it is the source of genes in cells. This experiment provides DNA extraction LyphoCells and reagents for isolating chromosomal DNA from <i>E. coli</i>. After spooling from solution, the DNA can be dissolved and analysed by agarose gel electrophoresis as an optional lab extension activity.

UOM: 1 * 1 KIT

Catalog Number: (BE-406)

Supplier: G-Biosciences

Description: Using the Protein Electrophoresis kit, students will learn the principles of various types of electrophoresis, including denaturing and non-denaturing electrophoresis, and how this powerful technique is used to analyse proteins. The kit will introduce students to the different separation matrices currently in use and will understand their differing separation properties and their role in protein analysis. Students have an option of casting their own electrophoresis gels using polyacrylamide or using pre-cast commercially available gels. This kit is provided with all of the reagents, buffers and supplies needed for casting acrylamide gels, preparing protein samples, running electrophoresis, and staining the gels for visualisation of protein bands. Test protein samples and protein standards are also provided.

UOM: 1 * 1 KIT

Supplier: EDVOTEK

Description: Ready-to-Load™ kit to show how electrophoresis separates molecules on the basis of size and charge.

Supplier: MP Biomedicals

Description: Dithioerythritol is an erythro isomer of 2,3-dihydroxy-1,4-dithiolbutane, and an isomer of 1,4-dithiothreitol which is a reagent for maintaining thiols in the reduced state; prevents oxidation of sulfhydryl-containing proteins during SDS-polyacrylamide gel electrophoresis. It also quantitatively reduces disulfides.

Supplier: MP Biomedicals

Description: Sodium 1-heptanesulfonate is a commonly used ion pairing reagent for HPLC, including analyses of peptides and proteins. It has also been used in high performance capillary electrophoresis analysis of peptides.

Catalog Number: (PIER24600)

Supplier: Thermo Scientific

Description: Pierce silver stain for mass spectrometry is a complete kit for rapid and ultra-sensitive silver staining of proteins in polyacrylamide gels and efficient destaining of excised gel pieces for mass spectrometry analysis. This kit enables both first-time and experienced users to achieve consistent and reliable staining using high, low, and gradient-percentage gels in 1D and 2D formats. The optimised staining method ensures extremely sensitive staining while minimizsing covalent crosslinking of protein to the gel matrix, which can inhibit protein recovery. The destaining reagents facilitate complete removal of silver from stained protein bands and maximum protein recovery for subsequent mass spectrometry analysis.

UOM: 1 * 1 KIT

Catalog Number: (786-411)

Supplier: G-Biosciences

Description: Insect PE LB™ has been developed for extraction of total biologically active, soluble proteins from adherent or suspension cultured insect cells, including Sf9 and Sf21. Insect PE LB™ utilises a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. The Insect PE LB™ is fully compatible with downstream processes, such as electrophoresis and chromatography. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Insect PE LB™.

UOM: 1 * 250 mL

Supplier: MP Biomedicals

Description: Tris and Tris Hydrochloride have been useful as buffers in a wide variety of biological systems. Uses include pH control<i> in vitro </i>and <i>in vivo</i> for body fluids and in buffering systems for electrophoresis applications.

Supplier: MP Biomedicals

Description: Storage: Store at Room Temperature (15 - 30 °C)
Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel’s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component. Glycerol is used in the concentration and storage of enzymes. Also prevents back-diffusion and protein samples into the buffer. Ideal for use as a cryoprotectant.

Catalog Number: (80-6484-51)

Supplier: Cytiva

Description: The 2-D Clean-Up Kit is designed to prepare samples for 2-D electrophoresis that otherwise produce poor 2-D spot-maps due to high conductivity, high levels of interfering substances, or low protein concentration. The reagents quantitatively precipitate proteins while leaving interfering substances, such as detergents, salts, lipids, phenolics, and nucleic acids, in solution.

UOM: 1 * 1 KIT

Catalog Number: (ICNA04856126)

Supplier: MP Biomedicals

Description: DL-Dithiothreitol is also known as Clelands reagent; Protective agent for sulfhydryl groups (-SH). Quantitatively reduces disulfides (-S-S- to -SH). In this reaction the DTT is oxidized to the cyclic disulfide which ensures the reduction of other disulfides in solution. Disulfide reduction occurs quickly at pH 8.
Dithiothreitol is useful for stabilizing sulfhydryl containing enzymes. Effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol.
Useful for stabilizing sulfhydryl-containing enzymes.

UOM: 1 * 5 g

Supplier: Thermo Scientific

Description: Thermo Scientific Pierce coomassie brilliant blue dyes are composed of one of the most common forms of coomassie dye, which is a key component of various colorimetric protein gel stains. Coomassie R-250 and G-250 dyes are two chemical forms of a disulphonated triphenylmethane compound that is commonly used as the basis of stains for detection of proteins in gel electrophoresis and bradford-type assay reagents for protein quantitation. The R-250 (red-tinted) form lacks two methyl groups that are present in the G-250 (green-tinted) form, which is also called colloidal coomassie dye.