You Searched For: Hybridisation+Buffers

Catalog Number: (MDTC46-021-CM)

Supplier: Corning Life Science

Description: 20X Saline-Sodium-Phosphate-EDTA (SSPE) buffer has applications in nucleic acid hybridization and other molecular biology work.

UOM: 1 * 6 items

Supplier: G-Biosciences

Description: Hyb-LINK™ is a high performance formamide-free hybridisation buffer solution for Northern, Southern and Dot Blots, which increases sensitivity of random-primer DNA probes.

Catalog Number: (KPLI50-86-05)

Supplier: SERACARE LIFE SCIENCES

Description: Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)

UOM: 1 * 1.000 mL

Catalog Number: (MDTC46-020-CM)

Supplier: Corning Life Science

Description: 20X Saline-Sodium Citrate (SSC) buffer is used in both nucleic acid hybridization and blot transfer procedures

UOM: 1 * 6 items

Certificates

Catalog Number: (733-1638)

Supplier: LONZA

Description: AccuGENE™ electrophoresis buffers are ready-to-use solutions ideal for a wide range of molecular biology applications.

UOM: 1 * 1 L

Catalog Number: (71978-3)

Supplier: Merck Millipore (Novagen)

Description: The One Step RT-PCR Master Mix Kit allows rapid, sensitive analysis of gene expression from tissues and cells. It can replace methods for detecting and quantifying gene expression such as Northern blots,<i> in situ</i> hybridisation, dot blots, S nuclease assays and conventional two step RT-PCR. The kit utilises recombinant <i>Thermus thermophilus</i> (rTth) DNA Polymerase, which acts as both a thermostable RNA dependent DNA polymerase and a DNA dependent DNA polymerase. The rTth DNA Polymerase is provided in a 2X master mix with an antibody for antibody-mediated hot start, optimised buffer, and ultrapure deoxynucleotides. Antibody-mediated Hot Start enhances specificity of both reverse transcription and PCR. The kit enables cDNA synthesis from input RNA followed by PCR amplification of the cDNA in a single reaction, with no additional hands-on requirement for buffer changes or adding reagents. Typically, detection of a specific transcript requires only 2 hours.

UOM: 1 * 1 KIT

Supplier: OMEGA BIO-TEK

Description: The Mag-Bind® Plant DNA 96 plus kit allows rapid and reliable isolation of high-quality genomic DNA from a wide variety of difficult to lyse and high polysaccharide plant species and tissues. The system utilises a CTAB-based lysis buffer without the need for a chloroform or other organic solvent steps. The proprietary wash buffer systems eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. This kit is designed for manual or fully automated high throughput preparation of genomic, chloroplast, and mitochondrial DNA. The Mag-Bind® Plant DNA plus kit also provides greater flexibility than silica plate based systems as partial plates can be used and can be scaled up to a 24-well format with the appropriate magnetic stand. The streamlined protocol can be easily adapted on most robotic liquid handling platforms. Purified DNA is suitable for PCR, qPCR, restriction digestion, hybridisation applications, as well as more demanding applications, such as RAPD.

MSDS

Catalog Number: (732-4300)

Supplier: UVP ULTRA VIOLET PRODUCTS

Description: The Hybridiser oven heats up fast and can be stacked to make the best use of available space. The variable speed control enables consistent saturation of samples for washing or hybridising and the carousel can be replaced with a rocker tray as an alternative, if purchased as an accessory.

UOM: 1 * 1 items

Catalog Number: (732-4301)

Supplier: UVP ULTRA VIOLET PRODUCTS

Description: The HybriLinker oven combines an HB-1000 hybridisation oven with a CX-2000 crosslinker with operation independent of one another. The crosslinker portion of the unit has a UV light emission at 254 nm for the bonding of DNA to a membrane in seconds. The UV dose can be entered as either time or microjoules per cm² of energy for accurate exposure.

UOM: 1 * 1 items

Catalog Number: (732-4315)

Supplier: UVP ULTRA VIOLET PRODUCTS

Description: The Minidizer oven is designed as a personal desk-top unit that is both compact and portable. It is ideal for use in start-up laboratories or those with limited space and low usage requirements. The unit cover swings open to allow easy access to the carousel, which can also be removed for easy changing of bottles.

UOM: 1 * 1 items

Catalog Number: (0507-500G)

Supplier: VWR Chemicals

Description: Common reagent for nucleic acid hybridisation procedures for detection of DNA/RNA.

UOM: 1 * 500 g

MSDS

Supplier: VWR Chemicals

Description: Ideal for denaturing nucleic acids in hybridisation and DNA sequencing procedures.

MSDS

Supplier: VWR Collection

Description: Microscope slides recommended for haematoxylin and eosin (H&E) stain and hybridisation on frozen sections in histopathology examination.

Catalog Number: (D9015-100MG)

Supplier: SIGMA ALDRICH MICROSCOPY

Description: 3,3′-Diaminobenzidine tetrahydrochloride hydrate has been used in immunohistological staining procedure and <i>in situ</i> hybridisation. DAB (3,3′-Diaminobenzidine) is utilised in many applications for the visualisation of peroxidase activity. In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidised DAB forms an insoluble brown end-product for use in immunohistological and immunoblotting staining procedures.

UOM: 1 * 100 mg

Supplier: UVP ULTRA VIOLET PRODUCTS

Description: Hybridisation bottles, large, Type: UVP, Borosilicate glass 3.3, Ø×L: 35×300 mm

Catalog Number: (1B1509-25G)

Supplier: VWR Chemicals

Description: DNA, salt, from fish sperm is an effective blocking agent used to increase signal-to-noise ratio for detection during hybridisation.

UOM: 1 * 25 g