You Searched For: Microarray+Plates

Supplier: GREINER BIO ONE

Description: Plastic plates for high-throughput microarrays. With 96 shallow wells and a low well rim, the HTA™ Plate has been optimised for the simultaneous analysis of multiple samples.

Supplier: OMEGA BIO-TEK

Description: The E-Z 96® Total RNA Kit is designed for isolation of total cellular RNA from up to 5×10⁶ cultured cells or soft tissues. This kit can process single or multiple samples in less than 60 minutes. Utilising HiBind® silica plate technology, the need for phenol/chloroform extractions, CsCl gradient ultracentrifugation, and precipitation with isopropanol or LiCl are eliminated. Samples are lysed in a denaturing lysis buffer which inactivates RNases. Binding conditions are adjusted and the lysate is transferred to a 96-well HiBind® RNA Plate where the RNA is purified via three wash steps. High quality RNA is eluted in RNase-free water. RNA purified using the E-Z 96® Total RNA method is ready for applications such as RT-PCR, qPCR, differential display, microarrays, and other downstream applications.

MSDS

Catalog Number: (MLDVGENEPIX4000B-U)

Supplier: Molecular Devices

Description: The GenePix® 4000B microarray scanner is a benchmark for quality, reliability and ease-of-use in microarray scanning technology. Coupled with GenePix® Pro microarray image analysis software and Acuity® microarray informatics software, the GenePix system sets the highest standards in the acquisition and analysis of data from all types of arrays, including nucleic acids, proteins, tissues, and cells.

UOM: 1 * 1 items

Supplier: Molecular Devices

Description: Red laser kit, 635 nm

Catalog Number: (MLDVGENEPIX4100A-U)

Supplier: Molecular Devices

Description: GenePix® 4100A Microarray Scanner offers its simplicity, reliability and flexibility in microarray-based research, be it in genomics, proteomics, or novel applications. It has all the quality, sensitivity, reliability and ease-of use of more expensive scanners, but in a price range and bench top footprint that makes it ideal for individual lab use.

UOM: 1 * 1 items

Supplier: Molecular Devices

Description: The GenePix® 4300A and GenePix® 4400A scanners offer high resolution, high quality imaging with 3-laser excitation. Configurations include 5 μm or 2,5 μm per-pixel maximum scanning resolution, choices of up to four lasers for excitation, and sixteen emission-wavelength filters.

Catalog Number: (40064.)

Supplier: Biotium

Description: Cyanine 555-dUTP can be used to synthesise labeled DNA probes for in-situ hybridization, microarray or blotting techniques.

UOM: 1 * 25 Nmo

Catalog Number: (S4651-72EA)

Supplier: SIGMA ALDRICH MICROSCOPY

Description: Silane-prep slides are microscope slides coated with silane (aminoalkysilane) to improve the adhesion of tissues to the slide for immunohistochemistry. The slide includes a 'frosted end' for slide/patient identification. It is not tested for use in microarray assays.

UOM: 1 * 72 items

Catalog Number: (40065.)

Supplier: Biotium

Description: Cyanine 647-dUTP can be used to synthesise labeled DNA probes for in-situ hybridization, microarray or blotting techniques.

UOM: 1 * 25 Nmo

Catalog Number: (40059.)

Supplier: Biotium

Description: DEAC-dUTP (diethylaminocoumarin-dUTP) can be used to synthesize fluorescently labeled DNA probes for in-situ hybridization, microarray or blotting techniques.

UOM: 1 * 25 Nmo

Supplier: AGILENT

Description: High-quality total RNA for human, mouse, and rat microarray gene-expression profiling that acts as a consistent control for standard data set comparisons.

Catalog Number: (COBSD7-000)

Supplier: Columbia Biosciences

Description: SureLight™ P3 is a proprietary dye can be used in prompt fluorescent detection, microplate applications, flow cytometry and protein microarrays.

UOM: 1 * 5 mg

Catalog Number: (NOVUNB120-11193)

Supplier: Novus Biologicals

Description: The Vinculin Antibody (VIN-11-5) from Novus Biologicals is a mouse monoclonal antibody to Vinculin. This antibody reacts with human, mouse, bovine, chicken. The Vinculin Antibody (VIN-11-5) has been validated for the following applications: Western Blot, Immunocytochemistry / Immunofluorescence, Microarray.

UOM: 1 * 0,1 mL

Catalog Number: (BOSSBS-1135R-A680)

Supplier: Bioss

Description: c-Src tyrosine kinase plays a critical role in signal transduction downstream of growth factor receptors, integrins and G protein-coupled receptors. We used stable isotope labelling with amino acids in cell culture (SILAC) approach to identify additional substrates of c-Src tyrosine kinase in human embryonic kidney 293T cells. We have identified 10 known substrates and interactors of c-Src and Src family kinases along with 26 novel substrates. We have experimentally validated 4 of the novel proteins (NICE-4, RNA binding motif 10, FUSE-binding protein 1 and TRK-fused gene) as direct substrates of c-Src using in vitro kinase assays and cotransfection experiments. Significantly, using a c-Src specific inhibitor, we were also able to implicate 3 novel substrates (RNA binding motif 10, EWS1 and Bcl-2 associated transcription factor) in PDGF signaling. Finally, to identify the exact tyrosine residues that are phosphorylated by c-Src on the novel c-Src substrates, we designed custom peptide microarrays containing all possible tyrosine-containing peptides (312 unique peptides) and their mutant counterparts containing a Tyr --> Phe substitution from 14 of the identified substrates. Using this platform, we identified 34 peptides that are phosphorylated by c-Src. We have demonstrated that SILAC-based quantitative proteomics approach is suitable for identification of substrates of nonreceptor tyrosine kinases and can be coupled with peptide microarrays for high-throughput identification of substrate phosphopeptides.

UOM: 1 * 100 µl

Catalog Number: (ROCKMB-045)

Supplier: Rockland Immunochemicals

Description: Saline-sodium citrate (SSC) buffer is most commonly used as a buffer for the hybridisation process and washing in experiments where spcies of nucleic or ribonucleic acid are to be matched to complimentary sequences (such as Southern and Nouthern blotting, <i>in situ</i> hybridisation, or DNA microarrays). SSC is used to help control the stringency of hybridisation in these steps.

UOM: 1 * 1 L

Catalog Number: (NOVUNB120-10454)

Supplier: Novus Biologicals

Description: The Caspase-11 Antibody (17D9) from Novus Biologicals is a rat monoclonal antibody to Caspase-11. This antibody reacts with human, mouse. The Caspase-11 Antibody (17D9) has been validated for the following applications: Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry / Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Microarray.

UOM: 1 * 0,1 mL