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Autoinduction systems, Overnight Express™ NMR Media

Supplier: Merck Millipore (Novagen)
Description: The Overnight Express™ Autoinduction Systems enable regulated protein expression in <i>E. coli</i>, without monitoring the culture or adding inducer during cell growth.

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KOD Hot start master mix

Supplier: Merck Millipore (Novagen)
Description: Premixed 2X KOD Hot Start PCR components for convenience and reproducibility.

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S-protein agarose, Novagen

Catalog Number: (69704-4)
Supplier: Merck Millipore (Novagen)
Description: S-protein agarose specifically retains STag™ fusion proteins. The capacity of S-protein agarose varies and is based on the size and folding characteristics of a given target protein.
UOM: 1 * 10 mL
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BCA protein assay kit

Catalog Number: (71285-3)
Supplier: Merck Millipore (Novagen)
Description: BCA Protein Assay is a simple and reliable protein quantification method. It can be used to determine protein concentration in the range of 20-2000 µg/ml in either a standard assay or microassay configuration.
UOM: 1 * 1 KIT
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Protein extraction reagent, BugBuster® (primary amine-free), Millipore®

Supplier: Merck Millipore (Novagen)
Description: BugBuster® (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilisation or cross-linking. The PIPPS buffer used in BugBuster® (primary amine-free) has a similar buffer capacity and pH range as the original Tris-buffered BugBuster®, but will not complex metal ions, also making it ideally suited for extraction of metal dependent proteins.

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D-Desthiobiotin

Catalog Number: (71610-3)
Supplier: Merck Millipore (Novagen)
Description: D-Desthiobiotin provides a gentle elution of StrepTag® II proteins from the biotin-binding site of StrepTactin® resins. D-Desthiobiotin is offered as a lyophilised powder for preparation of elution buffer.
UOM: 1 * 1 g
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Lysonase

Supplier: Merck Millipore (Novagen)
Description: Lysonase™ bioprocessing reagent is an optimised, ready-to-use convenient blend of rLysozyme™ solution and benzonase nuclease which is ideal for protein extraction.

MSDS

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Streptavidin agarose

Catalog Number: (69203-3)
Supplier: Merck Millipore (Novagen)
Description: Streptavidin agarose consists of cross-linked agarose covalently coupled with pure streptavidin under controlled conditions. The stable linkage to the resin minimizes leaching of the streptavidin while maintaining full binding activity. The matrix is suitable for use in column and batch formats for any application that requires high biotin binding capacity and low non-specific binding. The resin has no detectable protease, DNase, or RNase contamination.
UOM: 1 * 5 mL
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Autoinduction systems, Overnight Express™ Autoinduction System 1

Supplier: Merck Millipore (Novagen)
Description: The Overnight Express™ Autoinduction Systems enable regulated protein expression in <i>E. coli</i>, without monitoring the culture or adding inducer during cell growth.

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Protein extraction reagents, BugBuster® and BugBuster® plus Benzonase®, Millipore®

Supplier: Merck Millipore (Novagen)
Description: BugBuster® Protein Extraction reagent is formulated for the gentle disruption of the cell wall of <i>E. coli</i>, liberating soluble protein. It provides a simple, rapid, low-cost alternative to mechanical methods such as French Press or sonication for releasing expressed target protein in preparation for purification or other applications. The formulation utilises a detergent mix that is capable of cell wall perforation without denaturing soluble protein.

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Antibody labelling kits, HisTag® Antibody HRP Conjugate kit

Catalog Number: (71840-3)
Supplier: Merck Millipore (Novagen)
Description: The HisTag® Antibody HRP Conjugate kit contains a horseradish peroxidase-conjugated HisTag® Monoclonal Antibody that is useful as a single detection reagent, thus eliminating cross-reactivity associated with secondary antibody reagents. It recognises five consecutive histidine residues regardless of the surrounding amino acid context. The purified antibody conjugate produces a strong signal at a 1:1000 to 1:2000 dilution on Western blots and dot blots. The HisTag® Antibody HRP Conjugate also detects the recombinant marker bands in the Novagen Perfect Protein™ Western Markers for convenient visualisation of accurate internal standards on Western blots.
UOM: 1 * 125 µl
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Autoinduction systems, Overnight Express™ Instant LB and TB Media

Supplier: Merck Millipore (Novagen)
Description: The Overnight Express™ Autoinduction Systems enable regulated protein expression in <i>E. coli</i>, without monitoring the culture or adding inducer during cell growth.

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Anti-His tag Mouse Monoclonal Antibody

Supplier: Merck Millipore (Novagen)
Description: This His Tag® monoclonal antibody is a mouse monoclonal antibody (IgG1) directed against the His tag sequence encoded by many of the expression vectors. The antibody recognizes five consecutive histidine residues regardless of the surrounding amino acid context.

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DNA kit, BacMagic™-2

Catalog Number: (72157-3)
Supplier: Merck Millipore (Novagen)
Description: The BacMagic™-2 DNA Kit provides a positive selection for creating baculoviruses recombinants in preparation for generating recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification.
UOM: 1 * 1 KIT
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T7Select® phage display system, Novagen®

Supplier: Merck Millipore (Novagen)
Description: Novagen® T7Select® Phage Display System takes advantage of the properties of bacteriophage T7. This system is easy to use and has the capacity to display peptides up to about 50 amino acids in size in high copy number (415 per phage), and peptides or proteins up to about 1200 amino acids in low copy number (0,1 to 1 per phage) or mid-copy number (5 to 15 per phage).

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Thrombin (biotinylated)

Supplier: Merck Millipore (Novagen)
Description: Biotinylated Thrombin has covalently attached biotin for easy removal of the enzyme from cleavage reactions using immobilised streptavidin. This preparation is tested for activity using the same assay as for unmodified thrombin, and for >99% binding to streptavidin agarose.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 88 22222.
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