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Thrombin (biotinylated)

Supplier: Merck Millipore (Novagen)
Description: Biotinylated Thrombin has covalently attached biotin for easy removal of the enzyme from cleavage reactions using immobilised streptavidin. This preparation is tested for activity using the same assay as for unmodified thrombin, and for >99% binding to streptavidin agarose.

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Enterokinase cleavage capture kit

Catalog Number: (69067-3)
Supplier: Merck Millipore (Novagen)
Description: The Enterokinase Cleavage Capture Kit is designed for highly specific cleavage of fusion proteins followed by rapid, affinity-based capture and removal of enterokinase. Following cleavage of the target protein, rEK is removed with >99% efficiency from the reaction by affinity capture on EKapture™ Agarose. Following capture of rEK, the EKapture Agarose is removed by spin filtration, because the same buffer conditions are used for both cleavage and capture no buffer changes are necessary.
UOM: 1 * 1 KIT
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dNTP mix, 10 mM

Catalog Number: (71004-3)
Supplier: Merck Millipore (Novagen)
Description: The 10 mM dNTP mix is a ready-to-use preparation of ultrapure dATP, dCTP, dGTP, and dTTP (monosodium salts) at a concentration of 10 mm each in sterile deionised water at pH 7,0. Qualified for any application that requires pure deoxynucleotides, such as PCR, cDNA synthesis, and fill-in reactions.
UOM: 1 * 0,2 mL
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Autoinduction systems, Overnight Express™ Autoinduction System 2

Catalog Number: (71366-4)
Supplier: Merck Millipore (Novagen)
Description: The Overnight Express™ Autoinduction Systems enable regulated protein expression in <i>E. coli</i>, without monitoring the culture or adding inducer during cell growth.
UOM: 1 * 1 items
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Western blotting markers, Perfect Protein™, Novagen®

Catalog Number: (69959-3)
Supplier: Merck Millipore (Novagen)
Description: The Perfect Protein™ Western Markers consist of seven recombinant S•Tag™/His•Tag® fusion proteins that can be detected on any Western blot using an S-protein AP or HRP conjugate. As an additional option, the markers can also be detected with an His•Tag Monoclonal Antibody and an AP or HRP conjugated secondary antibody. The Perfect Protein Western Markers serve as precise size standards that appear simultaneously on the blot with target proteins, eliminating the uncertainty and imprecision associated with other methods.
UOM: 1 * 1 items
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KOD Xtreme™ Hot Start DNA polymerase

Catalog Number: (71975-3)
Supplier: Merck Millipore (Novagen)
Description: KOD Xtreme™ Hot Start DNA Polymerase is an ultra high fidelity DNA polymerase designed for the most challenging PCR applications, including crude sample PCR, amplification of long strand and highly GC-rich DNA templates.
UOM: 1 * 200 AU
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Ni-NTA His-Bind® Superflow™ Resin

Supplier: Merck Millipore (Novagen)
Description: Ni-NTA HisBind® Superflow™ Resin is a medium-pressure compatible version of the Ni-NTA HisBind® Resin

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Protein extraction reagent, BugBuster® HT, Millipore®

Supplier: Merck Millipore (Novagen)
Description: BugBuster® HT Protein Extraction Reagent combines BugBuster® Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster® HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from <i>E. coli</i> without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.

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Dialysers, D-Tube™ and D-Tube96™

Supplier: Merck Millipore (Novagen)
Description: D-Tube96™ Dialysers allow convenient, high throughput dialysis of 96 samples simultaneously. The device features the advantages of the D-Tube™ Dialyzer Mini in a 96-tube format. D-Tube Dialysers are easy to handle tubes with dual membranes providing a large surface area for fast, efficient dialysis. The membrane is ultra clean, EDTA-treated, regenerated cellulose that is sulfur and heavy metal-free.

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Perfect DNA™ markers

Supplier: Merck Millipore (Novagen)
Description: Molecular size markers for routine use in plasmid cloning, the Perfect DNA™ markers contain sets of DNA species having convenient, easy-to-remember sizes for agarose gel analysis. The markers have uniform band intensities except for the indicated reference bands, which are useful for instant band identification.

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DNA polymerases

Supplier: Merck Millipore (Novagen)
Description: KOD HiFi DNA Polymerase is a unique proofreading enzyme, isolated from the extreme thermophile <i>Thermococcus kodakaraensis</i> KOD1, which possesses superior processivity and fidelity that enables faster and more accurate PCR amplification than can be achieved with conventional enzymes.

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Novagen® GST Bind™ Resin, MilliporeSigma

Supplier: Merck Millipore (Novagen)
Description: GST Bind™ Resin utilizes an 14-atom spacer arm to covalently attach reduced glutathione via a sulfide linkage. The high degree of substitution of glutathione ensures a high binding capacity with yields of glutathione S-transferase (GST) fusion proteins of 5 to 8 mg/ml settled resin. The resin can be re-used up to 6 times without loss of capacity.

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Tris(hydroxypropyl)phosphine solution 0.5 M, Millipore®

Supplier: Merck Millipore (Novagen)
Description: THP [Tris(hydroxypropyl)phosphine; C9H21O3P] is a water-soluble, odorless, sulfhydryl reducing agent that is more stable and effective than dithiothreitol (DTT).

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PCR primers, Mouse Ig-primer set

Catalog Number: (69831-3)
Supplier: Merck Millipore (Novagen)
Description: The Mouse Ig-Primer Set is designed for amplification of immunoglobulin (Ig) variable region cDNAs. Mammalian light- and heavy-chain Igs contain conserved regions adjacent to the hypervariable complementarity defining regions (CDRs). Appropriately designed oligonucleotide primer sets enable these regions to be specifically amplified using PCR, after which the products may be sequenced directly or cloned.
UOM: 1 * 1 SET
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siRNA transfection reagent, RiboJuice™

Catalog Number: (71115-4)
Supplier: Merck Millipore (Novagen)
Description: RiboJuice™ siRNA Transfection Reagent delivers siRNA into a wide range of mammalian cell lines for targeted gene suppression. It is compatible with GeneJuice® Transfection Reagent for cotransfection of siRNA and plasmid DNA for normalising transfection efficiency with a reporter gene.
UOM: 1 * 1 mL
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Protein extraction reagent, BugBuster® 10X, Millipore®

Supplier: Merck Millipore (Novagen)
Description: BugBuster® 10X is a concentrated formulation of the proprietary detergents employed in BugBuster® without the addition of salts or buffer components. Concentrated BugBuster® provides a flexible alternative to the ready-to-use standard 1X BugBuster®, allowing user-defined dilution and addition of buffer components. BugBuster® 10X has all of the bioprocessing benefits of standard BugBuster® plus the freedom to control pH, reagent concentration, and buffer additives necessary for maximum extraction and activity of your target protein.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
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This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 88 22222.
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