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Supplier: MP Biomedicals
Description: Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C

Supplier: SIGMA ALDRICH MICROSCOPY
Description: Oil red O is a lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. In histology, a supersaturated solution of oil red O in isopropanol may be used to stain fat in tissue.

Supplier: VWR Chemicals
Description: Oil of castor, GPR RECTAPUR®
Catalog Number: (46949.)
Supplier: Merck
Description: Coconut oil
UOM: 1 * 1 items


Catalog Number: (1.13898.2500)
Supplier: Merck
Description: Standard oil, solvent for atomic absorption spectroscopy
UOM: 1 * 2,5 L


Supplier: VWR Chemicals
Description: Silicone oil, 20 cSt
Supplier: SIGMA ALDRICH MICROSCOPY
Description: Oil red O, Sigma-Aldrich®

Catalog Number: (47118.)
Supplier: Merck
Description: Olive oil
UOM: 1 * 1 items


Supplier: SIGMA ALDRICH MICROSCOPY
Description: Oil Blue N, Dye content 96% (Solvent Blue 14).

Supplier: MACRON AVANTOR BRAND
Description: Mineral oil heavy, white stabilised USP, Macron Fine Chemicals™
Catalog Number: (2705-01)
Supplier: Avantor
Description: Mineral oil, white stabilised USP, J.T.Baker®
UOM: 1 * 500 mL

Supplier: SIGMA ALDRICH MICROSCOPY
Description: Oil Red O stains lipid materials and imparts red-orange color to the lipids. It also marks vacuoles, particularly observed in ALL-L3 (acute lymphoblastic leukemia) or Burkitt′s lymphoma/leukemia. Oil Red O staining is done on fresh samples, as alcohol fixation removes lipids.

Supplier: Merck
Description: Silicone oil used as oil bath up to 250 °C.

Supplier: VWR Chemicals
Description: Silicone oil, GPR RECTAPUR® for oil baths up to 250°C
Supplier: MACRON AVANTOR BRAND
Description: Mineral oil light, white NF, Macron Fine Chemicals™
Catalog Number: (1.02419.0250)
Supplier: Merck
Description: Visualisation of neutral lipids in cryosections
UOM: 1 * 250 mL


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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
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