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Product Image-BSENBEK-2236-2P

proNGF Rapid™ ELISA Kit: Rat and Mouse

Supplier: Biosensis
Description: The Biosensis Mouse and Rat proNGF Rapid enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of rodent full-length proNGF protein in less than 4 hours in cell culture supernatants, cell lysates and tissue homogenates only if used as directed. This ELISA Kit has also successfully been used on mouse urine samples (Ryu JC et al., 2018). Please refer to the kit protocol for specific use instructions for each substrate application.

This ELISA kit contains a recombinant mouse proNGF standard expressed in E.coli and consists of a pre-coated anti-proNGF capture antibody, a biotinylated anti-proNGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of proNGF present in samples and protein standards. A proNGF positive control (QC sample) is provided to assure consistent assay performance. This ELISA kit detects rat proNGF due to high degree of homology (96%) with mouse proNGF based on amino acid sequence, and the ability of this kit in detecting proNGF in rat PC12 cell lysates and rat brain tissue homogenate. In the absence of a true rat proNGF standard, results may be expressed as 'mouse proNGF equivalents'. This ELISA kit shows only 20% reactivity with the human form of proNGF and is therefore not suitable to quantify human proNGF. No cross-reactivity was observed with mature mouse NGF and full-length proBDNF when tested in assay buffer.

The antibodies used in this ELISA kit bind epitopes within the pro-domain (capture) and mature domain (detection) of the protein, thus this ELISA assay does not detect the pro-domain peptide. This kit has not been tested for other applications. Sufficient amount of proNGF standard is supplied to allow for spike- and recovery experiments in order to validate this ELISA assay for other sample matrices if required.

Product Image-ACRO128590010

Nitro blue tetrazolium chloride monohydrate (NBT) 90%

Supplier: Thermo Scientific
Description: Nitro blue tetrazolium chloride monohydrate (NBT) 90%

Image Unavailable-APOSOR304398-5G

3-Methyl-3,9-diazaspiro[5.5]undecane dihydrochloride

Supplier: APOLLO SCIENTIFIC
Description: 3-Methyl-3,9-diazaspiro[5.5]undecane dihydrochloride

Image Unavailable-L07440.14

2-(Aminomethyl)benzimidazole dihydrochloride hydrate 98%

Supplier: Alfa Aesar
Description: 2-(Aminomethyl)benzimidazole dihydrochloride hydrate 98%

Image Unavailable-D2679-1VL

O-Dianisidine dihydrochloride, Supelco®

Catalog Number: (D2679-1VL)
Supplier: Merck
Description: O-Dianisidine dihydrochloride, Supelco®
UOM: 1 * 1 items
Image Unavailable-APOSOR304428-500MG

4-Oxa-1,9-diazaspiro[5.5]undecane dihydrochloride

Supplier: APOLLO SCIENTIFIC
Description: 4-Oxa-1,9-diazaspiro[5.5]undecane dihydrochloride

Image Unavailable-APOSOR304403-1G

2,9-Diazaspiro[5.5]undecane dihydrochloride

Catalog Number: (APOSOR304403-1G)
Supplier: APOLLO SCIENTIFIC
Description: 2,9-Diazaspiro[5.5]undecane dihydrochloride
UOM: 1 * 1 g
Product Image-ACRO227422500

Puromycin dihydrochloride hydrate 99%

Supplier: Thermo Scientific
Description: Puromycin dihydrochloride hydrate 99%

MSDS

Image Unavailable-APOSBIH127-25MG

H-89 dihydrochloride hydrate ≥99%

Supplier: APOLLO SCIENTIFIC
Description: Potent and selective inhibitor of PKA, with an IC50 of about 50 nM.  J. Biol. Chem. 265:  5267-5272 (1990)

Image Unavailable-D3252-100G

O-Dianisidine dihydrochloride ≥95%, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY
Description: o-Dianisidine (3,3′-dimethoxybenzidine) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is yellow-orange in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 5 M HCl.

Image Unavailable-F5803-50MG

O-Dianisidine dihydrochloride suitable for use in glucose determination, Sigma-Aldrich®

Catalog Number: (F5803-50MG)
Supplier: SIGMA ALDRICH MICROSCOPY
Description: A preweighed vial containing 50 mg o-Dianisidine. o-Dianisidine (3,3′-dimethoxybenzidine) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is yellow-orange in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 5 M HCl.
UOM: 1 * 50 mg
Image Unavailable-H63234.06

(2R,2'R)-Dimethyl-3,3'-disulphanediylbis(2-aminopropanoate) dihydrochloride 98%

Supplier: Alfa Aesar
Description: (2R,2'R)-Dimethyl-3,3'-disulphanediylbis(2-aminopropanoate) dihydrochloride 98%

Image Unavailable-APOSOR55003-5G

Pentan-3-ylhydrazine dihydrochloride

Supplier: APOLLO SCIENTIFIC
Description: Pentan-3-ylhydrazine dihydrochloride

Image Unavailable-APOSOR471615-100G

(2R,2'R)-Dimethyl-3,3'-disulphanediylbis(2-aminopropanoate) dihydrochloride 95%

Catalog Number: (APOSOR471615-100G)
Supplier: APOLLO SCIENTIFIC
Description: (2R,2'R)-Dimethyl-3,3'-disulphanediylbis(2-aminopropanoate) dihydrochloride 95%
UOM: 1 * 100 g
Product Image-ACRO463220050

Pramipexole dihydrochloride monohydrate ≥98%

Supplier: Thermo Scientific
Description: Pramipexole dihydrochloride monohydrate ≥98%

Image Unavailable-APOSOR15770-1G

3-Amino-6-azaindole dihydrochloride

Supplier: APOLLO SCIENTIFIC
Description: 3-Amino-6-azaindole dihydrochloride

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 88 22222.
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