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Hyclone™ Ham's F-10 Basal Media, Cell Culture Media, Liquid, Cytiva

Catalog Number: (HYCLSH30025.01)

Supplier: GE Healthcare - Hyclone

Description: Ham's F-10 was designed for use with Chinese Hamster Ovary (CHO) cells, and has been shown to support the growth of human diploid cells, white blood cells, and primary explants from rat, rabbit, or chicken tissues. This classical media can be used for serum-free growth of some cell lines including CHO cells. Serum supplementation may or may not be necessary depending on the type of cell being cultured. Ham's F-10 contains a wide variety of components, including zinc, hypoxanthine, and thymidine and is buffered with sodium bicarbonate.

UOM: 1 * 500 mL

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HyClone™ Ham's F-12 Basal Media, Cell Culture Media, Liquid

Supplier: GE Healthcare - Hyclone

Description: Ham's F-12 media was originally derived by modifying Ham's F-10 to include increased concentrations of choline, inositol, putrescine, and several amino acids. It was developed specifically for serum free culturing of Chinese Hamster Ovary (CHO) cells and has also been used with supplementation to grow chondrocytes, primary rat hepatocytes, rat prostate epithelial cells, carcinoma cells, rat skeletal myoblasts and rat, rabbit, and chicken embryos.

Eosin B, Certistain® for microscopy, Sigma-Aldrich®

Catalog Number: (1.15934.0025)

Supplier: Merck

Description: Eosin B, Certistain® for microscopy, Sigma-Aldrich®

UOM: 1 * 25 g

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Xylenol orange tetrasodium salt water_ACS reagent grade, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Description: Xylenol orange tetrasodium salt is a fluorochrome acid dye with complexing and chelating properties that make it useful as an indicator in the spectrophotometric detection and assay of metal ions such as calcium, cadmium, bismuth, zinc, lead, and mercury. Its tetrasodium salt especially is used as an indicator in metal titrations. It changes color from yellow to red between pH 6.4 to 10.4. It has six potentially anionic substituents, one being sulfonate, and is anionic under staining conditions and during most laboratory applications.

Phloxin B, Sigma-Aldrich®

Catalog Number: (P4030-25G)

Supplier: SIGMA ALDRICH MICROSCOPY

Description: Phloxine B has been used as a component of yeast extract peptone dextrose (YEPD) to grow Schizosaccharomyces pombe.

UOM: 1 * 25 g

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Phloxin B, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Description: Phloxine B has been used to trace the uptake of double stranded RNA by green peach aphids. It has also been used to stain yeast strains.

Cell culture media, Iscove's Modified Dulbecco's Medium (IMDM)

Supplier: BIOWEST

Description: In 1976, Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a reduced-serum medium supplemented with albumin, transferrin, lecithin, and selenium. Iscove's medium is a modification of Dulbecco's Modified Eagle's Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies have demonstrated that Iscove's medium will support murine B lymphocytes, haemopoietic tissue from bone marrow, B-cells stimulated with lipopolysaccharide, T-lymphocytes, and a variety of hybrid cells.