You Searched For: FERMENTAS

Supplier: Thermo Fisher Scientific

Description: The aLICator™ LIC Cloning and Expression System is designed for fast and efficient ligation independent cloning and tight regulation of gene expression in <i>E. coli</i>. The plate bacterial expression vectors are designed for high levels of target protein expression in concert with minimal basal (uninduced) expression, which permits expression of proteins that are toxic to <i>E. coli</i> cells.

Catalog Number: (ER0421)

Supplier: Thermo Fisher Scientific

Description: CCT sites and cuts best at 37 °C in B buffer (Isoschizomers: AatI, PceI, SseBI, StuI).

UOM: 1 * 1 KIT

Catalog Number: (ER1821)

Supplier: Thermo Fisher Scientific

Description: NGGCC sites and cuts best at 50 °C in G buffer.

UOM: 1 * 1 KIT

Catalog Number: (R0441)

Supplier: Thermo Fisher Scientific

Description: ATP (adenosine 5'-triphosphate) is an extremely stable nucleotide, and it is supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH. This nucleotide is used in different molecular biology applications, and it has greater than 99% purity.

UOM: 1 * 1 KIT

Supplier: Thermo Fisher Scientific

Description: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Supplier: Thermo Fisher Scientific

Description: DreamTaq™ PCR Master Mix (2X) is a ready to use solution containing DreamTaq™ DNA polymerase, optimised DreamTaq™ buffer, MgCl₂ and dNTPs. This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set-up. DreamTaq™ DNA polymerase is an enhanced <i>Taq</i> polymerase optimised for high throughput PCR applications. It ensures robust amplification of PCR products up to 6 kb from genomic DNA and up to 20 kb from viral DNA.

Supplier: Thermo Fisher Scientific

Description: DreamTaq Green PCR Master Mix (2X) is a ready to use solution containing DreamTaq™ DNA polymerase, optimised DreamTaq Green buffer, MgCl₂ and dNTPs. The master mix is supplemented with two tracking dyes and a density reagent that allows for direct loading of the PCR product on a gel. The dyes in the master mix do not interfere with PCR performance and are compatible with downstream applications, such as DNA sequencing, ligation and restriction digestion. The master mix retains all features of DreamTaq DNA polymerase. It is capable for robust amplification up to 6 kb from genomic DNA and up to 20 kb from viral DNA.

Supplier: Thermo Fisher Scientific

Description: Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix with dsDNase provides a simple workflow that combines genomic DNA elimination and cDNA synthesis in a one-tube procedure. The cDNA reaction components are pre-mixed into a complete master mix that is convenient to use, reduces pipetting steps, and is optimised for cDNA synthesis in two-step quantitative RT-PCR (RT-qPCR) applications. Maxima™ H Minus cDNA Synthesis Master Mix is optimised for reproducible cDNA synthesis at elevated temperatures (50 to 65°C) within 30 minutes.

Catalog Number: (SM1821)

Supplier: Thermo Fisher Scientific

Description: RiboRuler™ high range RNA ladder allows precise sizing of RNA molecules in denaturing agarose gels and Northern blotting.

UOM: 1 * 1 KIT

Supplier: Thermo Fisher Scientific

Description: GeneJET Gel Extraction Kit is designed for rapid and efficient purification of DNA fragments from standard or low-melting point agarose gels run in either TAE or TBE buffer.

Supplier: Thermo Fisher Scientific

Description: The Maxima™ SYBR® Green/Fluorescein qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start Taq DNA polymerase and dNTPs in an optimised PCR buffer. It contains SYBR® Green I dye and is supplemented with fluorescein passive reference dye. Fluorescein is included into the master mix to serve as an internal reference for normalisation
of the SYBR Green I fluorescent signal between different wells to compensate for any instrument or pipetting variation. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG)*. Maxima™ hot start Taq DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. The use of Maxima™ SYBR® Green/Fluorescein qPCR Master Mix in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

Supplier: Thermo Fisher Scientific

Description: Maxima™ Reverse Transcriptase is an advanced enzyme derived by <i>in vitro</i> evolution of M-MuLV RT for RT-qPCR application. The enzyme features high thermostability, robustness and increased synthesis rates in RT-qPCR. The Maxima™ Reverse Transcriptase is capable of cDNA synthesis at elevated temperatures (50 to 60 °C) and completion of the reverse transcription reaction in 15 to 30 minutes. The use of this enzyme in combination with Maxima™ qPCR Master Mixes ensures high specificity, sensitivity and wide linear range of two step RT-qPCR. Applications include first strand cDNA synthesis, RT-PCR, and RT-qPCR.

Catalog Number: (SM1103)

Supplier: Thermo Fisher Scientific

Description: FastRuler ultra low range DNA ladder is specifically designed for fast sizing and approximate quantification of double-stranded DNA in 48 well (or 96-well) high throughput gels, as well as in conventional agarose gels. The ladder is a mixture of five blunt-ended chromatography-purified individual DNA fragments that are easily resolved in a short separation distance (10 to 20 mm) after an 8 to 14 minutes run on the appropriate agarose gel.

UOM: 1 * 1 KIT

Catalog Number: (SM0383)

Supplier: Thermo Fisher Scientific

Description: MassRuler Low Range DNA Ladder, ready to use, 80 to 1031 bp, is specially designed for accurate quantification and sizing of DNA fragments by agarose gel electrophoresis.

UOM: 1 * 1 KIT

Catalog Number: (ER1751)

Supplier: Thermo Fisher Scientific

Description: CCNGGA sites and cuts best at 37 °C in Tango buffer.

UOM: 1 * 1 KIT

Supplier: Thermo Fisher Scientific

Description: Catalyses the transfer of the γ-phosphate from ATP to the 5'-hydroxyl group of single- and double-stranded DNAs and RNAs, oligonucleotides, or nucleoside 3'-monophosphates (forward reaction).