You Searched For: Novagen (Part of Merck)

Supplier: Merck Millipore (Novagen)

Description: Ni-NTA HisBind® Resin is a high-performance Ni²⁺-charged agarose used for rapid one-step purification of proteins containing a HisTag® sequence by metal chelation chromatography.

Catalog Number: (69660-3)

Supplier: Merck Millipore (Novagen)

Description: The pET-14b vector carries an N-terminal His·Tag® sequence followed by a thrombin cleavage site and three cloning sites.

UOM: 1 * 10 µG

Catalog Number: (70607-3)

Supplier: Merck Millipore (Novagen)

Description: Loading Buffer, 4X SDS sample buffer

UOM: 1 * 2 mL

Catalog Number: (70852-3)

Supplier: Merck Millipore (Novagen)

Description: The SpinPrep™ Gel DNA Kit enables efficient extraction of DNA fragments of 150 bp to >12000 bp in size from agarose gels. The procedure uses GelMelt™ solution to dissolve the gel slice, followed by adsorption of the DNA to a silica membrane in a spin column format. After a wash step, the purified DNA is eluted in low salt buffer. Each spin column can bind up to 20 µg DNA. Recoveries are consistently in the range of 50 to 90% under standard conditions.

UOM: 1 * 100 Tests

Catalog Number: (69203-3)

Supplier: Merck Millipore (Novagen)

Description: Streptavidin agarose consists of cross-linked agarose covalently coupled with pure streptavidin under controlled conditions. The stable linkage to the resin minimizes leaching of the streptavidin while maintaining full binding activity. The matrix is suitable for use in column and batch formats for any application that requires high biotin binding capacity and low non-specific binding. The resin has no detectable protease, DNase, or RNase contamination.

UOM: 1 * 5 mL

Supplier: Merck Millipore (Novagen)

Description: This His Tag® monoclonal antibody is a mouse monoclonal antibody (IgG1) directed against the His tag sequence encoded by many of the expression vectors. The antibody recognizes five consecutive histidine residues regardless of the surrounding amino acid context.

Catalog Number: (69671-3)

Supplier: Merck Millipore (Novagen)

Description: Restriction grade thrombin is a highly purified preparation qualified to specifically cleave target proteins produced with appropriate vectors. This preparation is functionally tested for activity with fusion proteins and is free of detectable contaminating proteases.

UOM: 1 * 50 EU

Catalog Number: (69067-3)

Supplier: Merck Millipore (Novagen)

Description: The Enterokinase Cleavage Capture Kit is designed for highly specific cleavage of fusion proteins followed by rapid, affinity-based capture and removal of enterokinase. Following cleavage of the target protein, rEK is removed with >99% efficiency from the reaction by affinity capture on EKapture™ Agarose. Following capture of rEK, the EKapture Agarose is removed by spin filtration, because the same buffer conditions are used for both cleavage and capture no buffer changes are necessary.

UOM: 1 * 1 KIT

Catalog Number: (69182-3)

Supplier: Merck Millipore (Novagen)

Description: Desoxyribonuclease I

UOM: 1 * 1.000 EU

Catalog Number: (69278-3)

Supplier: Merck Millipore (Novagen)

Description: PCR Molecular Size Marker contains a mixture of eight defined DNA fragments ranging from 50 to 2000 bp at convenient size intervals for characterising small DNA products. The markers are supplied in gel loading buffer containing two tracking dyes that do not interfere with UV illumination of ethidium bromide-stained bands. A separate vial of 6X Loading Buffer is included. Each vial of markers is sufficient for 50 lanes on TBE or TAE agarose stained with ethidium bromide. The recommended amount for loading (5 µl) produces bands of even intensity that are bright, sharp and easy to photograph.

UOM: 1 * 1 items

Supplier: Merck Millipore (Novagen)

Description: BugBuster® (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilisation or cross-linking. The PIPPS buffer used in BugBuster® (primary amine-free) has a similar buffer capacity and pH range as the original Tris-buffered BugBuster®, but will not complex metal ions, also making it ideally suited for extraction of metal dependent proteins.

Catalog Number: (71013-4)

Supplier: Merck Millipore (Novagen)

Description: ColiRollers™ plating beads are a specially treated glass bead which offers a better way to perform plating bacteria for transformation and molecular cloning applications. ColiRollers™ beads eliminate the inconsistencies of conventional 'hockey stick' methods.

UOM: 1 * 5 SET

MSDS

Catalog Number: (72157-3)

Supplier: Merck Millipore (Novagen)

Description: The BacMagic™-2 DNA Kit provides a positive selection for creating baculoviruses recombinants in preparation for generating recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification.

UOM: 1 * 1 KIT

Catalog Number: (71759-4)

Supplier: Merck Millipore (Novagen)

Description: Overnight Express™ Autoinduction NMR Medium, 5 L kit

UOM: 1 * 4 SET

Supplier: Merck Millipore (Novagen)

Description: Insect GeneJuice® Transfection Reagent is formulated to maximise transfection efficiency of Sf9 insect cells. The reagent features extremely low toxicity to the cells and can be used for both transient and stable transfections in serum-containing or serum-free media, and for co-transfection of transfer plasmids with linearised virus DNA for the production of recombinant baculoviruses.

Supplier: Merck Millipore (Novagen)

Description: BugBuster® master mix combines BugBuster® protein extraction reagent with Benzonase® Nuclease and rLysozyme™ solution in one convenient reagent. It allows for maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. The master mix provides sufficient reagents for protein extraction from 20 g and 100 g cell paste.