You Searched For: Antibody+Purification+Buffers

Supplier: OMEGA BIO-TEK

Description: The E.Z.N.A.® bacterial DNA kit allows the rapid and reliable isolation of high quality total cellular DNA from a wide variety of bacterial species. This kit uses optimised lysis condition and up to 1×10⁹ bacterial cells can be processed for each column. There are no organic extractions, thus reducing plastic waste and hands on time to allow multiple samples to be processed in parallel. Bacterial cells are grown to log-phase and harvested. The cell wall is removed by lysozyme digestion and bead beating, followed by protease digestion. Following lysis, binding conditions are adjusted and the sample is applied to a HiBind DNA spin-column. Two rapid wash steps remove trace salts and protein contaminants, and DNA is finally eluted in water or elution buffer. Purified DNA can be directly used in downstream applications without the need for further purification.

MSDS

Supplier: Cytiva

Description: Sephadex G-50 grade F is a size exclusion chromatography resin designed for desalting and buffer exchange, and for purification of DNA and oligonucleotides greater than 20 bases in length using spin columns.

MSDS

Catalog Number: (70976-3)

Supplier: Merck Millipore (Novagen)

Description: The SpinPrep™ PCR Clean Up Kit is designed for the rapid purification of DNA amplified by PCR. The 10-minute procedure involves addition of a binding buffer followed by adsorption of the DNA to a silica membrane in a spin column format. Following a wash step, the DNA is eluted in low salt buffer. This kit removes DNA polymerises, dNTPs, salts, and >99% of primers so that they do not interfere with downstream applications such as cloning, sequencing, or labelling. PCR products from 100 to >12000 bp can be cleaned up, with recoveries of amplified DNA in the range of 60 to 90% under standard conditions.

UOM: 1 * 100 Tests

Supplier: MP Biomedicals

Description: GeneClean® Turbo Kit is used for purification of DNA fragments of sizes 0,1 kb to 300 kb from TAE or TBE buffered agarose gels, PCR reactions and other enzymatic solutions. Purified DNA can be used in enzymatic reactions, conventional or automated sequencing, cloning and microarray analysis.

Catalog Number: (28-9034-45)

Supplier: Cytiva

Description: The illustra™ GFX™ 96 Purification Kit utilises glass fibre matrix technology in a 96-well format for highly efficient purification of PCR products. Fragments from PCR are captured by the matrix in the presence of a chaotropic salt, and contaminants are conveniently removed by washing the matrix with a buffered ethanol solution.

UOM: 1 * 1 KIT

Supplier: Biotium

Description: The DNA Gel Extraction Kit is a silica-gel, spin column based DNA extraction kit designed to purify 40 bp to 40 kb DNA fragments from agarose gels in TAE or TBE buffer.

MSDS

Supplier: Thermo Fisher Scientific

Description: GeneJET Gel Extraction Kit is designed for rapid and efficient purification of DNA fragments from standard or low-melting point agarose gels run in either TAE or TBE buffer.

MSDS

Catalog Number: (GZ-02)

Supplier: G-Biosciences

Description: The EZ Yeast™ Plasmid Prep Kit is designed for the rapid isolation of plasmids from yeast patches or yeast grown in small liquid cultures. The kit is adapted from the alkaline lysis of <i>E. coli</i>, by providing modified alkaline lysis buffers and LongLife™ Zymolyase®, a highly stable enzyme.

UOM: 1 * 1 KIT

Catalog Number: (ICNA111001400)

Supplier: MP Biomedicals

Description: The GeneClean® II Kit is designed to optimally purify 200 bp to 20 kb DNA from TAE and TBE buffered agarose gels and solutions. The purified DNA is suitable for a variety of downstream applications, such as sequencing, PCR, restriction digestion, cloning and labeling.

UOM: 1 * 1 KIT

Supplier: BARNSTEAD THERMOLYNE

Description: The Barnstead™ E-Pure™ is designed to connect directly to the tap water feed, and it can handle all of the laboratory high volume ultrapure water applications with low operating costs. This system is ideal for AA, GS, buffer preps, or as a purified feed water source for analytical instruments or labware washers.

Supplier: OMEGA BIO-TEK

Description: The E.Z.N.A.® DNA/RNA isolation kit is designed for the simultaneous isolation of both genomic DNA and total RNA from the same cells or tissues. The sample is first lysed and homogenised in a special denaturing buffer, spun to pellet RNA and undigested particles, and then the supernatant is applied to a HiBind® DNA spin column to bind DNA. The RNA pellet is dissolved and purified by a HiBind RNA spin column. Since there is no need to divide the sample into two parts for separate purification procedures, maximum yield of DNA and RNA can be purified from the entire sample.

MSDS Certificates

Catalog Number: (27-3565-01)

Supplier: Cytiva

Description: MicroSpin™ columns are designed for the rapid purification of DNA in conjunction with Sephadex™ G-25 DNA grade for use in a wide range of applications, including desalting, buffer exchange and removal of unincorporated nucleotides from end-labelled oligonucleotides.

UOM: 1 * 1 items

Supplier: G-Biosciences

Description: GET™ AGAROSE DNA method involves release of DNA fragments from gel pieces followed by capture of nucleic acids on the GET™ spin columns, washing, and elution of the clean nucleic acid fragments in a suitable buffer.

Catalog Number: (ICNA116075050)

Supplier: MP Biomedicals

Description: FastRNA™ Pro Soil-Indirect Kit is used to isolate total RNA from organic material found in soil samples. This kit allows for the sample to be slurried with water or buffer in order to further separate biomass from the soil or environmental matrix, and the resulting supernatant is then filtered, lysed, and purified through the rest of the protocol. Total RNA isolated is suitable for RT-PCR analysis and other downstream applications.

UOM: 1 * 50 Tests

Catalog Number: (E406-5ML)

Supplier: VWR Chemicals

Description: Ethidium bromide solution is a fluorescent stain for visualising nucleic acids in solution or in electrophoresis gels. The solution is provided at a concentration of 0,625 mg/ml. A final working concentration of 0,5 g/ml is easily obtained by adding one drop of solution to 50 ml of molten agarose, running buffer or staining buffer. Ethidium bromide solution in dropper bottles may be stored at room temperature for up to one year.

UOM: 1 * 5 mL

MSDS

Supplier: BARNSTEAD THERMOLYNE

Description: This system is a modularly designed laboratory reverse osmosis system specifically developed for functional and economical production of high purity water. Ideal for routine laboratory work: Rinsing lab glassware, supplying autoclaves, water baths and incubators, preparing and diluting buffers and reagents and general biotechnology.