You Searched For: Novagen (Part of Merck)

Supplier: Merck Millipore (Novagen)

Description: This His Tag® monoclonal antibody is a mouse monoclonal antibody (IgG1) directed against the His tag sequence encoded by many of the expression vectors. The antibody recognizes five consecutive histidine residues regardless of the surrounding amino acid context.

Catalog Number: (69671-3)

Supplier: Merck Millipore (Novagen)

Description: Restriction grade thrombin is a highly purified preparation qualified to specifically cleave target proteins produced with appropriate vectors. This preparation is functionally tested for activity with fusion proteins and is free of detectable contaminating proteases.

UOM: 1 * 50 EU

Catalog Number: (69067-3)

Supplier: Merck Millipore (Novagen)

Description: The Enterokinase Cleavage Capture Kit is designed for highly specific cleavage of fusion proteins followed by rapid, affinity-based capture and removal of enterokinase. Following cleavage of the target protein, rEK is removed with >99% efficiency from the reaction by affinity capture on EKapture™ Agarose. Following capture of rEK, the EKapture Agarose is removed by spin filtration, because the same buffer conditions are used for both cleavage and capture no buffer changes are necessary.

UOM: 1 * 1 KIT

Catalog Number: (69182-3)

Supplier: Merck Millipore (Novagen)

Description: Desoxyribonuclease I

UOM: 1 * 1.000 EU

Catalog Number: (69278-3)

Supplier: Merck Millipore (Novagen)

Description: PCR Molecular Size Marker contains a mixture of eight defined DNA fragments ranging from 50 to 2000 bp at convenient size intervals for characterising small DNA products. The markers are supplied in gel loading buffer containing two tracking dyes that do not interfere with UV illumination of ethidium bromide-stained bands. A separate vial of 6X Loading Buffer is included. Each vial of markers is sufficient for 50 lanes on TBE or TAE agarose stained with ethidium bromide. The recommended amount for loading (5 µl) produces bands of even intensity that are bright, sharp and easy to photograph.

UOM: 1 * 1 items

Supplier: Merck Millipore (Novagen)

Description: BugBuster® (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilisation or cross-linking. The PIPPS buffer used in BugBuster® (primary amine-free) has a similar buffer capacity and pH range as the original Tris-buffered BugBuster®, but will not complex metal ions, also making it ideally suited for extraction of metal dependent proteins.

Catalog Number: (71013-4)

Supplier: Merck Millipore (Novagen)

Description: ColiRollers™ plating beads are a specially treated glass bead which offers a better way to perform plating bacteria for transformation and molecular cloning applications. ColiRollers™ beads eliminate the inconsistencies of conventional 'hockey stick' methods.

UOM: 1 * 5 SET

MSDS

Catalog Number: (72157-3)

Supplier: Merck Millipore (Novagen)

Description: The BacMagic™-2 DNA Kit provides a positive selection for creating baculoviruses recombinants in preparation for generating recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification.

UOM: 1 * 1 KIT

Catalog Number: (71759-4)

Supplier: Merck Millipore (Novagen)

Description: Overnight Express™ Autoinduction NMR Medium, 5 L kit

UOM: 1 * 4 SET

Supplier: Merck Millipore (Novagen)

Description: The Overnight Express™ Autoinduction Systems enable regulated protein expression in <i>E. coli</i>, without monitoring the culture or adding inducer during cell growth.

Catalog Number: (69078-3)

Supplier: Merck Millipore (Novagen)

Description: The Perfect Protein™ HRP Western Blot Kit includes an HRP or AP conjugate in addition to Perfect Protein™ Western markers.

UOM: 1 * 1 items

Catalog Number: (71115-4)

Supplier: Merck Millipore (Novagen)

Description: RiboJuice™ siRNA Transfection Reagent delivers siRNA into a wide range of mammalian cell lines for targeted gene suppression. It is compatible with GeneJuice® Transfection Reagent for cotransfection of siRNA and plasmid DNA for normalising transfection efficiency with a reporter gene.

UOM: 1 * 1 mL

Supplier: Merck Millipore (Novagen)

Description: Molecular size markers for routine use in plasmid cloning, the Perfect DNA™ markers contain sets of DNA species having convenient, easy-to-remember sizes for agarose gel analysis. The markers have uniform band intensities except for the indicated reference bands, which are useful for instant band identification.

Supplier: Merck Millipore (Novagen)

Description: The Overnight Express™ Autoinduction Systems enable regulated protein expression in <i>E. coli</i>, without monitoring the culture or adding inducer during cell growth.

Supplier: Merck Millipore (Novagen)

Description: Premixed 2X KOD Hot Start PCR components for convenience and reproducibility.

Supplier: Merck Millipore (Novagen)

Description: A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.