You Searched For: Novagen (Part of Merck)

Supplier: Merck Millipore (Novagen)

Description: D-Tube96™ Dialysers allow convenient, high throughput dialysis of 96 samples simultaneously. The device features the advantages of the D-Tube™ Dialyzer Mini in a 96-tube format. D-Tube Dialysers are easy to handle tubes with dual membranes providing a large surface area for fast, efficient dialysis. The membrane is ultra clean, EDTA-treated, regenerated cellulose that is sulfur and heavy metal-free.

Catalog Number: (71281-3)

Supplier: Merck Millipore (Novagen)

Description: ProteoJuice™ protein transfection reagent is an effective reagent for the transfection of intact functional protein and peptides into mammalian cells with minimal toxicity and broad cell specificity.

UOM: 1 * 1 items

Catalog Number: (69704-4)

Supplier: Merck Millipore (Novagen)

Description: S-protein agarose specifically retains STag™ fusion proteins. The capacity of S-protein agarose varies and is based on the size and folding characteristics of a given target protein.

UOM: 1 * 10 mL

Supplier: Merck Millipore (Novagen)

Description: BugBuster® HT Protein Extraction Reagent combines BugBuster® Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster® HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from <i>E. coli</i> without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.

Supplier: Merck Millipore (Novagen)

Description: The pSTBlue-1 AccepTor™ vector kits are designed to simplify the PCR cloning process by using non-proofreading thermostable DNA polymerases (eg. KOD XL polymerase and native and recombinant <i>Taq</i> polymerases), which leave single 3'-dA overhangs on the reaction products. The AccepTor™ vectors enable direct ligation by virtue of single 3'-dU overhangs that anneal efficiently with 3'-dA extensions on PCR products. The dU residues are converted to dT residues<i> in vivo</i> following transformation.

Catalog Number: (70856-3)

Supplier: Merck Millipore (Novagen)

Description: Convenient solution for selective degradation of RNA.

UOM: 1 * 1 mL

Supplier: Merck Millipore (Novagen)

Description: BugBuster® Plus Lysonase™ includes BugBuster Protein Extraction Reagent and Lysonase Bioprocessing Reagent, an optimised, ready-to-use blend of rLysozyme™ and Benzonase® Nuclease in a single kit. This powerful combination of two reagents allows maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. The BugBuster® and rLysozyme™ efficiently lyse the bacterial cells while the Benzonase® degrades nucleic acids to facilitate downstream processing. Requires 5 ml BugBuster and 10 µl Lysonase per 1 g cell paste. The kits provide sufficient reagents for protein extraction from 20 g or 100 g cell paste.

Catalog Number: (70527-3)

Supplier: Merck Millipore (Novagen)

Description: The lac operon inducer IPTG (isopropyl-β-D- 1-thiogalactopyranoside) is recommended for blue/white screening by lacZ α- complementation with appropriate vectors and host strains, such as pSTBlue-1/NovaBlue, and for inducing protein expression with the pET System and other lac promoter-derived expression systems.

UOM: 1 * 15 mL

Catalog Number: (69750-3)

Supplier: Merck Millipore (Novagen)

Description: The pET-24a-d(+) vectors carry an N-terminal T7-Tag® sequence plus an optional C-terminal His-Tag® sequence. These vectors differ from pET-21a-d(+) only by their selectable marker (kanamycin vs ampicillin resistance).

UOM: 1 * 10 µG

Catalog Number: (69755-3)

Supplier: Merck Millipore (Novagen)

Description: The His·Bind buffer kit is a set of pre-tested buffers designed for use with IDA-based His·Bind resin for convenient, rapid one-step purification of proteins by metal chelation chromatography.

UOM: 1 * 1 KIT

Supplier: Merck Millipore (Novagen)

Description: NovaBlue competent cells are designed for convenient and reliable plasmid transformation. NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue/white screening capability (with appropriate plasmids), and <i>recA</i> and <i>endA</i> mutations, which result in high yields of excellent-quality plasmid DNA. Standard NovaBlue and convenient NovaBlue Singles™ are ideal for routine cloning, while NovaBlue GigaSingles™ are most suitable for applications requiring higher transformation efficiencies.

Supplier: Merck Millipore (Novagen)

Description: A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Supplier: Merck Millipore (Novagen)

Description: Pellet Paint® Co-Precipitant is a visible dye-labelled carrier formulated specifically for use in alcohol precipitation of nucleic acids. The 2-minute precipitation does not require low-temperature incubations or prolonged centrifugation. Both RNA and DNA are efficiently precipitated from even the most dilute solutions (2 ng/ml) and the pellet is easily located by its vivid pink colour. The pellet can be easily followed during washing steps and this prevents losses during handling and ensures thorough pellet re-suspension.

Catalog Number: (71004-3)

Supplier: Merck Millipore (Novagen)

Description: The 10 mM dNTP mix is a ready-to-use preparation of ultrapure dATP, dCTP, dGTP, and dTTP (monosodium salts) at a concentration of 10 mm each in sterile deionised water at pH 7,0. Qualified for any application that requires pure deoxynucleotides, such as PCR, cDNA synthesis, and fill-in reactions.

UOM: 1 * 0,2 mL

Catalog Number: (72181-3)

Supplier: Merck Millipore (Novagen)

Description: 293-Free™ transfection reagent has a unique polycationic liposomal formulation and is designed expressly for transfection of HEK 293 cells grown in suspension culture. It is ideal for mammalian protein production. 293-Free™ transfection reagent is provided as a sterile, ready to use solution. The 1 ml size provides enough reagent to transfect a litre of culture.

UOM: 1 * 1 mL

Supplier: Merck Millipore (Novagen)

Description: BugBuster® Protein Extraction reagent is formulated for the gentle disruption of the cell wall of <i>E. coli</i>, liberating soluble protein. It provides a simple, rapid, low-cost alternative to mechanical methods such as French Press or sonication for releasing expressed target protein in preparation for purification or other applications. The formulation utilises a detergent mix that is capable of cell wall perforation without denaturing soluble protein.